Microelectrodes fabrication using laser scissor

Today laser microdissection system or laser scissor is frequently used for isolation of single chromosome, nucleus, cell, tissue etc. Here we fabricated the gold microelectrodes having a gap spacing of less than 3 μm using this system. A 500 Å thickness of gold was coated on standard glass slide. A UV-laser (λ 337 nm) of 4 ns pulse duration having an energy of 22 μJ is sufficient to cold ablate the gold, was used for fabrication of gold electrodes on the above slides. Microstructures up to the resolution limit (0.25 μm) of optical microscope can be fabricated using this cold ablation by laser.