Microbial inhibition and biosensing with multifunctional carbon dots: Progress and perspectives

Carbon dots (CDs) and their doped counterparts including nitrogen-doped CDs (N@CDs) have been synthesized by bottom-up or top-down approaches from different precursors. The attractiveness of such emerging 2D‑carbon-based nanosized materials is attributed to their excellent biocompatibility, preparation, aqueous dispersibility, and functionality. The antimicrobial, optical, and electrochemical properties of CDs have been advocated for two important biotechnological applications: bacterial eradication and sensing/biosensing. CDs as well as N@CDs act as antimicrobial agents as their surfaces encompass functional hydroxyl, carboxyl, and amino groups that generate free radicals. As a new class of photoluminescent nanomaterials, CDs can be employed in diversified analytics. CDs with surface carboxyl or amino groups form nanocomposites with nanomaterials or be conjugated with biorecognition molecules toward the development of sensors/biosensors. The deployment of conductive CDs in electrochemical sensing has also increased significantly because of their quantum size, excellent biocompatibility, enzyme-mimicking activity, and high surface area. The review also addresses the ongoing challenges and promises of CDs in pathogenesis and analytics. Perspectives on the future possibilities include the use of CDs in microbial viability assay, wound healing, antiviral therapy, and medical devices.