TY - JOUR
T1 - Mice Developing Mammary Tumors Evolve T Cell Sequences Shared With Human Breast Cancer Patients
AU - Efroni, S.
AU - Gordin, Miri
AU - Philip, Hagit
AU - Zilberberg, Alona
AU - Gidoni, Moriah
AU - Margalit, Raanan
AU - Clouser, Christopher
AU - Adams, Kristofor
AU - Vigneault, Francois
AU - Irun, Cohen R.
AU - Yaari, Gur
AU - others, null
PY - 2018/8/6
Y1 - 2018/8/6
N2 - Cancer immunotherapy by checkpoint blockade proves that an effective immune response to a tumor can be induced clinically. However, little is known about the evolution of tumor-associated T-cell receptor (TCR) repertoires without intervention. Here we studied TCR repertoire evolution in mice spontaneously developing mammary tumors; we sequenced peripheral blood alpha and beta TCRs of CD4+CD62L+CD44− T cells monthly for 8 months in 10 FVB/NJ mice transgenic at the Erbb2 locus, all developing tumors; 5 FVB/NJ mice without the transgene were age-matched controls. Sequences were either private (restricted to one mouse) or public (shared among mice); public sequences were either exclusive to the tumor group or inclusive among different groups. We now report that 1), public AA sequences were each encoded by many different nucleotide sequences (NT) recombinations (convergent recombination; CR); 2) mice developing tumors evolved tumor-exclusive public sequences, derived initially from private or from inclusive public sequences; and 3) tumor-exclusive public sequences in mice were also present among published public TCR sequences from human breast cancer patients. These cross-species tumor-exclusive TCR sequences manifested high CR; but the AA sequences shared by mice and humans did not share NT sequences. Thus, tumor-exclusive TCR AA sequences across species are selected from different NT recombination events. The roles of tumor-exclusive TCR repertoires in advancing or inhibiting tumor development and the effects of tumor immunotherapy on these T cells remain to be seen.
AB - Cancer immunotherapy by checkpoint blockade proves that an effective immune response to a tumor can be induced clinically. However, little is known about the evolution of tumor-associated T-cell receptor (TCR) repertoires without intervention. Here we studied TCR repertoire evolution in mice spontaneously developing mammary tumors; we sequenced peripheral blood alpha and beta TCRs of CD4+CD62L+CD44− T cells monthly for 8 months in 10 FVB/NJ mice transgenic at the Erbb2 locus, all developing tumors; 5 FVB/NJ mice without the transgene were age-matched controls. Sequences were either private (restricted to one mouse) or public (shared among mice); public sequences were either exclusive to the tumor group or inclusive among different groups. We now report that 1), public AA sequences were each encoded by many different nucleotide sequences (NT) recombinations (convergent recombination; CR); 2) mice developing tumors evolved tumor-exclusive public sequences, derived initially from private or from inclusive public sequences; and 3) tumor-exclusive public sequences in mice were also present among published public TCR sequences from human breast cancer patients. These cross-species tumor-exclusive TCR sequences manifested high CR; but the AA sequences shared by mice and humans did not share NT sequences. Thus, tumor-exclusive TCR AA sequences across species are selected from different NT recombination events. The roles of tumor-exclusive TCR repertoires in advancing or inhibiting tumor development and the effects of tumor immunotherapy on these T cells remain to be seen.
UR - http://scholar.google.com/scholar?num=3&hl=en&lr=&q=allintitle%3A%20Mice%20developing%20mammary%20tumors%20evolve%20T%20cell%20sequences%20shared%20with%20human%20breast%20cancer%20patients%2C%20author%3AEfroni%20OR%20author%3AMiri%20OR%20author%3AGordin%20OR%20author%3AHagit%20OR%20author%3APhilip%20OR%20author%3AAlona%20OR%20author%3AZilberberg%20OR%20author%3AMoriah%20OR%20author%3AGidoni%20OR%20author%3ARaanan%20OR%20author%3AMargalit%20OR%20author%3AChristopher%20OR%20author%3AClouser%20OR%20author%3AKristofor%20OR%20author%3AAdams%20OR%20author%3AFrancois%20OR%20author%3AVigneault%20OR%20author%3AIrun%20OR%20author%3ACohen%20OR%20author%3AGur%20OR%20author%3AYaari%20OR%20author%3Aothers&as_ylo=2018&as_yhi=&btnG=Search&as_vis=0
U2 - 10.1101/371260
DO - 10.1101/371260
M3 - Article
SN - 2692-8205
SP - 1
EP - 10
JO - bioRxiv
JF - bioRxiv
M1 - 371260
ER -
