Methods to determine the role of autophagy proteins in C. elegans aging

Sivan Henis-Korenblit, Alicia Meléndez

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


This chapter describes methods for the analysis of autophagy proteins in C. elegans aging. We discuss the strains to be considered, the methods for the delivery of double-stranded RNA, and the methods to measure autophagy levels, autophagic flux, and degradation by autophagy.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages26
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
Work in the Henis-Korenblit laboratory is supported by grants: no. 2013188 from the Binational Science Foundation, no. I-1211-309.13/2012 from the German-Israeli Foundation for Scientific Research and Development, and no. 3-12066 from the Israeli Ministry of Science, Technology and Space, and work in the Meléndez Lab is supported by grants R15GM102846 from the National Institute of Health, a PSC-CUNY Research award, and A.M. was an Ellison Medical Foundation New Scholar in Aging (AG-NS-0521–0).

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.


  • Aging
  • Autophagy
  • Caenorhabditis elegans
  • Double-stranded RNA
  • GFP::LGG-1
  • HT115
  • L4
  • Longevity
  • RNA interference
  • SQST-1::GFP
  • Transgene
  • dsRNA


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