Abstract
This chapter describes methods for the analysis of autophagy proteins in C. elegans aging. We discuss the strains to be considered, the methods for the delivery of double-stranded RNA, and the methods to measure autophagy levels, autophagic flux, and degradation by autophagy.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 561-586 |
| Number of pages | 26 |
| DOIs | |
| State | Published - 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1880 |
| ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media, LLC, part of Springer Nature 2019.
Funding
Work in the Henis-Korenblit laboratory is supported by grants: no. 2013188 from the Binational Science Foundation, no. I-1211-309.13/2012 from the German-Israeli Foundation for Scientific Research and Development, and no. 3-12066 from the Israeli Ministry of Science, Technology and Space, and work in the Meléndez Lab is supported by grants R15GM102846 from the National Institute of Health, a PSC-CUNY Research award, and A.M. was an Ellison Medical Foundation New Scholar in Aging (AG-NS-0521–0).
| Funders | Funder number |
|---|---|
| National Institutes of Health | |
| National Institute of General Medical Sciences | R15GM102846 |
| Ellison Medical Foundation | |
| German-Israeli Foundation for Scientific Research and Development | I-1211-309.13/2012 |
| Ministry of Science, Technology and Space | 3-12066 |
Keywords
- Aging
- Autophagy
- Caenorhabditis elegans
- Double-stranded RNA
- GFP::LGG-1
- HT115
- L4
- Longevity
- RNA interference
- SQST-1::GFP
- Transgene
- dsRNA