Abstract
MeCP2, the major causative factor of Rett syndrome and related phenotypes including autism, is a two-face nuclear modulator acting via transcriptional and chromatin remodeling mechanisms. This study investigated the expression of several nuclear proteins and their dependence on MeCP2 dose and presence of the Rett causative R306C mutation. To this end, we developed in vitro models representing MeCP2 deficiency induced by siRNAs, and cells expressing the R306C mutation. Using an extended antibody microarray validated by specific assays, revealed that MeCP2 dose was correlated with specific nuclear proteins profiles including the BRM/SNF2 component of SWI/SNF complex, PRMT1 methyl transferase and HDAC2. Furthermore, while exposing the MeCP2 knock-down system to therapeutic concentrations of valproic acid (VPA), a known HDACs inhibitor, we observed a partial restoration of MeCP2 expression levels. Exposure to VPA also increased the levels of BRM, as well as of BDNF, an important co-factor in MeCP2-mediated pathway. Our findings provide additional evidence of diverse mechanisms of MeCP2 function as transcriptional repressor and activator of specific genes. as it has been recently demonstrated that post-natal restoration of MeCP2 deficiency may reverse neurological defects in a mouse model of Rett syndrome, we suggest to study the restorative effect of HDAC inhibitors in MeCP2-deficient mouse model.
Original language | English |
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Pages (from-to) | 61-67 |
Number of pages | 7 |
Journal | Epigenetics |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jan 2010 |
Externally published | Yes |
Bibliographical note
Funding Information:We thank Prof. Karen B. Avraham for her active support and for reviewing this article. We also thank Prof. Anat Biegon and Dr. Bruria Ben Zeev for constructive comments on this study. This work has been supported by the European Network on Rett Syndrome (EuroRETT) and partially supported by the Smith Laboratory for Collaborative Research, Life Science Institute, Hebrew University, Jerusalem, Israel.
Funding
We thank Prof. Karen B. Avraham for her active support and for reviewing this article. We also thank Prof. Anat Biegon and Dr. Bruria Ben Zeev for constructive comments on this study. This work has been supported by the European Network on Rett Syndrome (EuroRETT) and partially supported by the Smith Laboratory for Collaborative Research, Life Science Institute, Hebrew University, Jerusalem, Israel.
Funders | Funder number |
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European Network on Rett Syndrome | |
Hebrew University of Jerusalem |
Keywords
- Chromatin remodeling
- Gene expression
- HDAC inhibitors
- Methyl CpG binding protein 2 (MeCP2)
- Rett syndrome