TY - JOUR
T1 - Measuring cell proliferation in the rectal mucosa
T2 - Comparing bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA) assays
AU - Kulldorff, Martin
AU - McShane, Lisa M.
AU - Schatzkin, Arthur
AU - Freedman, Laurence S.
AU - Wargovich, Michael J.
AU - Woods, Cindy
AU - Purewal, Madhu
AU - Burt, Randall W.
AU - Lawson, Michael
AU - Mateski, Donna J.
AU - Lanza, Elaine
AU - Corle, Donald K.
AU - O'Brien, Barbara
AU - Moler, James
PY - 2000/8
Y1 - 2000/8
N2 - Cell proliferation in the human colorectum can be measured using bromodeoxyuridine (BrdU) or proliferating cell nuclear antigen (PCNA) assays. Using data from the National Cancer Institute's Polyp Prevention Trial, these two assays are compared using correlation coefficients and variance components analysis. Adjusting for fixed as well as for the random effects of between-biopsy and scoring variation, the estimated correlation is 0.46 for the log labeling index and 0.45 for log proliferative height. This is an estimate of the highest correlation that can be achieved by taking multiple biopsies scored by multiple scorers. For single biopsies, the estimated correlation is 0.16 and 0.10, respectively. There are significant differences between the variance components for the two assays. For example, for log labeling index, PCNA has a lower variation between biopsies than BrdU, but higher variation between scorings. When used in a clinical or epidemiological setting, it is important to take multiple biopsies at multiple time points. Copyright (C) 2000 Elsevier Science Inc.
AB - Cell proliferation in the human colorectum can be measured using bromodeoxyuridine (BrdU) or proliferating cell nuclear antigen (PCNA) assays. Using data from the National Cancer Institute's Polyp Prevention Trial, these two assays are compared using correlation coefficients and variance components analysis. Adjusting for fixed as well as for the random effects of between-biopsy and scoring variation, the estimated correlation is 0.46 for the log labeling index and 0.45 for log proliferative height. This is an estimate of the highest correlation that can be achieved by taking multiple biopsies scored by multiple scorers. For single biopsies, the estimated correlation is 0.16 and 0.10, respectively. There are significant differences between the variance components for the two assays. For example, for log labeling index, PCNA has a lower variation between biopsies than BrdU, but higher variation between scorings. When used in a clinical or epidemiological setting, it is important to take multiple biopsies at multiple time points. Copyright (C) 2000 Elsevier Science Inc.
KW - Biopsies
KW - Colon adenomas
KW - Colorectal cancer
KW - Correlation
KW - Variance components
UR - http://www.scopus.com/inward/record.url?scp=0033865947&partnerID=8YFLogxK
U2 - 10.1016/S0895-4356(99)00180-8
DO - 10.1016/S0895-4356(99)00180-8
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C2 - 10942872
AN - SCOPUS:0033865947
SN - 0895-4356
VL - 53
SP - 875
EP - 883
JO - Journal of Clinical Epidemiology
JF - Journal of Clinical Epidemiology
IS - 8
ER -
