Abstract
The development and homeostasis of multicellular organisms rely on coordinated regulation of cell migration. Cell migration is an essential event in the construction and regeneration of tissues, and is critical in embryonic development, immunological responses, and wound healing. Dysregulation of cell motility contributes to pathological disorders, such as chronic inflammation and cancer metastasis. Cell migration, tissue invasion, axon, and dendrite outgrowth all initiate with actin polymerization-mediated cell-edge protrusions. Here, we describe a simple, efficient, time-saving method for the imaging and quantitative analysis of cell-edge protrusion dynamics during spreading. This method measures discrete features of cell-edge membrane dynamics, such as protrusions, retractions, and ruffles, and can be used to assess how manipulations of key actin regulators impact cell-edge protrusions in diverse contexts.
| Original language | English |
|---|---|
| Article number | e63157 |
| Journal | Journal of Visualized Experiments |
| Volume | 2021 |
| Issue number | 177 |
| DOIs | |
| State | Published - 1 Nov 2021 |
Bibliographical note
Publisher Copyright:© 2021 JoVE Journal of Visualized Experiments.
Funding
This work was supported by grants NIH MH115939, NS112121, NS105640, and R56MH122449-01A1 (to Anthony J. Koleske) and from the Israel Science Foundation (grants number 1462/17 and 2142/21) (to Hava Gil-Henn).
| Funders | Funder number |
|---|---|
| National Institutes of Health | R56MH122449-01A1, NS105640, NS112121 |
| National Institute of Mental Health | R01MH115939 |
| Israel Science Foundation | 2142/21, 1462/17 |
