Measuring and mitigating PCR bias in microbiota datasets

Keyphrases

• 16S Ribosomal RNA (16S rRNA) 100%
• Bacterial Community 50%
• High-throughput DNA Sequencing 50%
• Human Gut Microbiota 50%
• Log-ratio 50%
• Microbial Abundance 50%
• Microbiota 100%
• Mixed Microbial Communities 50%
• PCR Amplification 50%
• PCR Bias 100%
• Primer Mismatch 50%

Immunology and Microbiology

• DNA Sequence 14%
• Intestine Flora 14%
• Microflora 100%
• Polymerase Chain Reaction 100%
• RNA Gene 14%

Medicine and Dentistry

• DNA Sequence 14%
• Gut Microbiota 14%
• Polymerase Chain Reaction 100%
• RNA 16S 28%
• RNA Gene 14%