Measurement of genetic dissimilarity in fieldpea (Pisum sativum L.) genotypes using RAPD markers

Vinod Kumar Yadav; Sandeep Kumar; Ravindra K. Panwar

doi:10.1007/s10722-006-9109-1

Measurement of genetic dissimilarity in fieldpea (Pisum sativum L.) genotypes using RAPD markers

Vinod Kumar Yadav, Sandeep Kumar, Ravindra K. Panwar

G.B. Pant University of Agriculture and Technology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken, out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic similarities of the RAPD profiles were estimated by using Jaccard's coefficient with NTSYSpc 2.0 software. The similarity index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes and major Group B have nine genotypes.

Original language	English
Pages (from-to)	1285-1289
Number of pages	5
Journal	Genetic Resources and Crop Evolution
Volume	54
Issue number	6
DOIs	https://doi.org/10.1007/s10722-006-9109-1
State	Published - Sep 2007
Externally published	Yes

Keywords

DNA isolation
Fieldpea
Germplasm
Pisum sativum
RAPD

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10.1007/s10722-006-9109-1

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abstract = "The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken, out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic similarities of the RAPD profiles were estimated by using Jaccard's coefficient with NTSYSpc 2.0 software. The similarity index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes and major Group B have nine genotypes.",

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AU - Yadav, Vinod Kumar

AU - Kumar, Sandeep

AU - Panwar, Ravindra K.

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Y1 - 2007/9

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AB - The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken, out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic similarities of the RAPD profiles were estimated by using Jaccard's coefficient with NTSYSpc 2.0 software. The similarity index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes and major Group B have nine genotypes.

KW - DNA isolation

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KW - Germplasm

KW - Pisum sativum

KW - RAPD

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Measurement of genetic dissimilarity in fieldpea (Pisum sativum L.) genotypes using RAPD markers

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