Mandibular defect repair by TGF-β and IGF-1 released from a biodegradable osteoconductive hydrogel

Samer Srouji, Adi Rachmiel, Israel Blumenfeld, Erella Livne

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Purpose: Bone regeneration is crucial in the healing of defects in the craniofacial complex. The ability of transforming growth factor-β1 (TGF-β1) and insulin-like growth factor-1 (IGF-1), incorporated into a hydrogel scaffold to induce bone regeneration, was evaluated in a rat mandible defect model. Material and methods: Hydrogel scaffolds containing either transforming growth factor-β1 (TGF-β1), insulin-like growth factor-1 (IGF-1), TGF-β+ IGF-1, or saline, were implanted in rat mandibular bone defects. In a control group the defects were treated by saline alone. Bone defect healing was tested after 3 and 6 weeks by radiology and morphology. Results: Soft tissue radiographs indicated that the area of new bone formation increased gradually after 3 and at 6 weeks. The percentage of closure after 3 weeks was less than the percentage closure after 6 weeks. The amount of calcified material in the TGF-β and TGF-β+ IGF-1-treated groups had increased more than in the saline-containing hydrogel and control (saline-treated) defects. The percentages of defect closures were 37, 38, 24, 14, and 11% after 3 weeks, and 94, 91, 84, 72, and 29% after 6 weeks, in the TGF-β+IGF-1, TGF-β, IGF-1, saline containing hydrogel and saline-treated animals, respectively. Three-dimensional computerized tomography (3D CT) images showed that the 3D shape of the bones was restored. Morphological analysis of the defects treated with hydrogel containing TGF-β, IGF-1 or TGF-β+IGF-1 revealed significant bone formation after 6 weeks. Conclusion: It is concluded that the hydrogel scaffold impregnated with growth factors can induce bone regeneration and is therefore a promising surgical tool for enhancement of surgical repair of bone defects.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalJournal of Cranio-Maxillofacial Surgery
Volume33
Issue number2
DOIs
StatePublished - Apr 2005
Externally publishedYes

Bibliographical note

Funding Information:
This work has been supported by Technion VPR grants Nos: 180-532; 180-405. The authors wish to express their thanks to Mrs. Pessia Shenzer and Mr. S. Weizman for excellent technical assistance and to Nitta Gelatin, Co. Osaka, Japan for supplying the hydrogel.

Funding

This work has been supported by Technion VPR grants Nos: 180-532; 180-405. The authors wish to express their thanks to Mrs. Pessia Shenzer and Mr. S. Weizman for excellent technical assistance and to Nitta Gelatin, Co. Osaka, Japan for supplying the hydrogel.

FundersFunder number
Technion-Israel Institute of Technology180-532, 180-405

    Keywords

    • Biodegradable hydrogel
    • Bone defect
    • Growth factors
    • Osteoconduction
    • Scaffold

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