Magnetic micro-device for manipulating PC12 cell migration and organization

N. Alon, T. Havdala, H. Skaat, K. Baranes, M. Marcus, I. Levy, S. Margel, A. Sharoni, O. Shefi

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Directing neuronal migration and growth has an important impact on potential post traumatic therapies. Magnetic manipulation is an advantageous method for remotely guiding cells. In the present study, we have generated highly localized magnetic fields with controllable magnetic flux densities to manipulate neuron-like cell migration and organization at the microscale level. We designed and fabricated a unique miniaturized magnetic device composed of an array of rectangular ferromagnetic bars made of permalloy (Ni80Fe20), sputter-deposited onto glass substrates. The asymmetric shape of the magnets enables one to design a magnetic landscape with high flux densities at the poles. Iron oxide nanoparticles were introduced into PC12 cells, making the cells magnetically sensitive. First, we manipulated the cells by applying an external magnetic field. The magnetic force was strong enough to direct PC12 cell migration in culture. Based on time lapse observations, we analysed the movement of the cells and estimated the amount of MNPs per cell. We plated the uploaded cells on the micro-patterned magnetic device. The cells migrated towards the high magnetic flux zones and aggregated at the edges of the patterned magnets, corroborating that the cells with magnetic nanoparticles are indeed affected by the micro-magnets and attracted to the bars' magnetic poles. Our study presents an emerging method for the generation of pre-programmed magnetic micro-'hot spots' to locate and direct cellular growth, setting the stage for implanted magnetic devices.

Original languageEnglish
Pages (from-to)2030-2036
Number of pages7
JournalLab on a Chip
Volume15
Issue number9
DOIs
StatePublished - 7 May 2015

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry 2015.

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