Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro

Alessandro Genna; Francisco W. Puerta Martinez; John C. McAuliffe; Dianne Cox

doi:10.1007/978-1-0716-4901-5_17

Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro

Alessandro Genna
, Francisco W. Puerta Martinez
, John C. McAuliffe
, Dianne Cox

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Macrophages orchestrate multiple functions in the tumor microenvironment leading to metastatic progression including promotion of tumor cell invasion, intravasation, and extravasation at metastatic sites. In vitro assays have been developed to explore these processes in high resolution. Here we describe an in vitro protocol used to examine macrophage enhanced tumor cell extravasation and intravasation in 3D using a stratified co-culture transendothelial migration (TEM) transwell system. These assays may be generally applied in order to study different aspect of metastatic dissemination, and we show here usefulness with breast and pancreatic tumor cell lines.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	193-204
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-0716-4901-5_17
State	Published - 2026
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2983
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.

Keywords

Macrophages
Microscopy
Transendothelial migration (TEM)
Tumor cell

Access to Document

10.1007/978-1-0716-4901-5_17

Cite this

@inbook{2ded02892ad14904bfa90cdd33ec5515,

title = "Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro",

abstract = "Macrophages orchestrate multiple functions in the tumor microenvironment leading to metastatic progression including promotion of tumor cell invasion, intravasation, and extravasation at metastatic sites. In vitro assays have been developed to explore these processes in high resolution. Here we describe an in vitro protocol used to examine macrophage enhanced tumor cell extravasation and intravasation in 3D using a stratified co-culture transendothelial migration (TEM) transwell system. These assays may be generally applied in order to study different aspect of metastatic dissemination, and we show here usefulness with breast and pancreatic tumor cell lines.",

keywords = "Macrophages, Microscopy, Transendothelial migration (TEM), Tumor cell",

author = "Alessandro Genna and \{Puerta Martinez\}, \{Francisco W.\} and McAuliffe, \{John C.\} and Dianne Cox",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.",

year = "2026",

doi = "10.1007/978-1-0716-4901-5\_17",

language = "אנגלית",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "193--204",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro

AU - Genna, Alessandro

AU - Puerta Martinez, Francisco W.

AU - McAuliffe, John C.

AU - Cox, Dianne

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.

PY - 2026

Y1 - 2026

N2 - Macrophages orchestrate multiple functions in the tumor microenvironment leading to metastatic progression including promotion of tumor cell invasion, intravasation, and extravasation at metastatic sites. In vitro assays have been developed to explore these processes in high resolution. Here we describe an in vitro protocol used to examine macrophage enhanced tumor cell extravasation and intravasation in 3D using a stratified co-culture transendothelial migration (TEM) transwell system. These assays may be generally applied in order to study different aspect of metastatic dissemination, and we show here usefulness with breast and pancreatic tumor cell lines.

AB - Macrophages orchestrate multiple functions in the tumor microenvironment leading to metastatic progression including promotion of tumor cell invasion, intravasation, and extravasation at metastatic sites. In vitro assays have been developed to explore these processes in high resolution. Here we describe an in vitro protocol used to examine macrophage enhanced tumor cell extravasation and intravasation in 3D using a stratified co-culture transendothelial migration (TEM) transwell system. These assays may be generally applied in order to study different aspect of metastatic dissemination, and we show here usefulness with breast and pancreatic tumor cell lines.

KW - Macrophages

KW - Microscopy

KW - Transendothelial migration (TEM)

KW - Tumor cell

UR - https://www.scopus.com/pages/publications/105026519923

U2 - 10.1007/978-1-0716-4901-5_17

DO - 10.1007/978-1-0716-4901-5_17

M3 - ???researchoutput.researchoutputtypes.contributiontobookanthology.chapter???

C2 - 41478978

AN - SCOPUS:105026519923

T3 - Methods in Molecular Biology

SP - 193

EP - 204

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Macrophage Enhancement of Tumor Cell Extravasation and Intravasation In Vitro

Abstract

Publication series

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this