Liver micro-organs transcribe albumin and clotting factors and increase survival of 92% hepatectomized rats

Etty Grad-Itach, Alicia Graciela Fuchs, Haya Lev, Tatyana Kotok, Ronen Shemesh, Daniel Shouval, Yaron Ilan, Eduardo Mitrani

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Background/Aims: Currently there is no effective non-surgical therapy for most patients with fulminant or end stage chronic liver disease. Methods: We have prepared rat liver micro-organs (LMOs), which preserve the liver micro-architecture and ensure that no cell is more than 150 μm away from a source of nutrients and gases. The function of LMOs has been evaluated in vitro and in a new extra-corporeal liver device termed aLIVE in which LMOs are exposed to liver-like hemodynamic conditions. Results: In vitro LMOs maintain normal physiological and biochemical functions including oxygen consumption, glucose metabolism, conversion of ammonia to urea, secretion of albumin and de novo transcription of genes coding for albumin and clotting factors. Inside the aLIVE bioreactor, LMOs also display sustained oxygen consumption, glucose metabolism and transcription of albumin and clotting factors IX and X, when connected both to normal and to 92% hepatectomized rats. Survival of 92% hepatectomized rats was 40% longer following a single 4-h treatment with aLIVE, compared to untreated animals. Conclusions: An extra-corporeal liver device, aLIVE, which provides key liver functions, has been developed. When tested in 92% hepatectomized rats, aLIVE improved the clinical condition and significantly increased survival time of the treated rats.

Original languageEnglish
Pages (from-to)552-558
Number of pages7
JournalJournal of Hepatology
Issue number4
StatePublished - 1 Oct 2003
Externally publishedYes


  • Albumin
  • Artificial liver support
  • Bioreactor
  • Clotting factor
  • Liver device
  • Liver failure


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