Lipoxygenase activity in heart cells

Eyal Breitbart; Yossi Sofer; Asher Shainberg; Shlomo Grossman

doi:10.1016/0014-5793(96)01017-4

Lipoxygenase activity in heart cells

Eyal Breitbart, Yossi Sofer, Asher Shainberg, Shlomo Grossman

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[¹⁴C]AA, LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

Original language	English
Pages (from-to)	148-152
Number of pages	5
Journal	FEBS Letters
Volume	395
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)01017-4
State	Published - 21 Oct 1996

Keywords

12-HETE
15-HETE
Cardiac muscle
Cardiomyocyte
Lipoxygenase

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10.1016/0014-5793(96)01017-4

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title = "Lipoxygenase activity in heart cells",

abstract = "Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA, LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.",

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AU - Breitbart, Eyal

AU - Sofer, Yossi

AU - Shainberg, Asher

AU - Grossman, Shlomo

PY - 1996/10/21

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N2 - Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA, LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

AB - Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA, LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

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KW - 15-HETE

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Lipoxygenase activity in heart cells

Abstract

Keywords

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