Lipoxygenase activity in heart cells

Eyal Breitbart, Yossi Sofer, Asher Shainberg, Shlomo Grossman

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1-[14C]AA, LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE. Enzyme linked immunosorbent assay for 12-HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15-HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalFEBS Letters
Volume395
Issue number2-3
DOIs
StatePublished - 21 Oct 1996

Keywords

  • 12-HETE
  • 15-HETE
  • Cardiac muscle
  • Cardiomyocyte
  • Lipoxygenase

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