Lipidomics Analyses Reveal Temporal and Spatial Lipid Organization and Uncover Daily Oscillations in Intracellular Organelles

Rona Aviram, Gal Manella, Naama Kopelman, Adi Neufeld-Cohen, Ziv Zwighaft, Meytar Elimelech, Yaarit Adamovich, Marina Golik, Chunyan Wang, Xianlin Han, Gad Asher

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

Cells have evolved mechanisms to handle incompatible processes through temporal organization by circadian clocks and by spatial compartmentalization within organelles defined by lipid bilayers. Recent advances in lipidomics have led to identification of plentiful lipid species, yet our knowledge regarding their spatiotemporal organization is lagging behind. In this study, we quantitatively characterized the nuclear and mitochondrial lipidome in mouse liver throughout the day, upon different feeding regimens, and in clock-disrupted mice. Our analyses revealed potential connections between lipid species within and between lipid classes. Remarkably, we uncovered diurnal oscillations in lipid accumulation in the nucleus and mitochondria. These oscillations exhibited opposite phases and readily responded to feeding time. Furthermore, we found that the circadian clock coordinates the phase relation between the organelles. In summary, our study provides temporal and spatial depiction of lipid organization and reveals the presence and coordination of diurnal rhythmicity in intracellular organelles. Aviram et al. performed lipidomics analyses of organelles from mouse liver to explore temporal and spatial principles in lipid organization. They find that the lipid composition of the nucleus and mitochondria oscillates daily with distinct and opposite phases. These oscillations respond to feeding time and are coordinated by the circadian clock.

Original languageEnglish
Pages (from-to)636-648
Number of pages13
JournalMolecular Cell
Volume62
Issue number4
DOIs
StatePublished - 19 May 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Funding

We are grateful to S. Itzkovitz and all the members the G.A. laboratory for their advice and valuable comments on the manuscript. Work in the G.A. lab was supported by the Israel Science Foundation (ISF 138/12) and European Research Council (ERC-2011 METACYCLES 310320). G.A. is a recipient of the EMBO Young Investigator Award and incumbent of the Pauline Recanati Career Development Chair. Work in the X.H. lab was partly supported by National Institute of General Medical Sciences grant R01 GM105724, American Diabetes Association, and intramural institutional research funds.

FundersFunder number
American Diabetes Association
National Institute of General Medical SciencesR01GM105724
European Research CouncilERC-2011 METACYCLES 310320
Israel Science FoundationISF 138/12

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