Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways

Lena Hazanov; Ramit Mehr; Yu Chang Bryan Wu; Deborah K. Dunn-Walters

doi:10.1109/AISW.2015.7469231

Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways

Lena Hazanov, Ramit Mehr, Yu Chang Bryan Wu, Deborah K. Dunn-Walters

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

King's College London

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

Transitional (TR) B cells are immature B cells that have migrated from the bone marrow to peripheral lymphoid organs, but can still undergo selection against autoreactivity. TR cells that survive selection eventually develop into mature naïve B cells (CD27-IgD+, NA). Upon exposure to antigen, NA cells may become IgM memory (CD27+IgD+, MM) or "classical", classswitched memory (CD27+IgD-, SM) B cells. Although MM immunoglobulin (Ig) genes do not undergo class switching, they do undergo somatic hypermutation, albeit with lower frequency than SM. It has been postulated that MM B cells originate from T-independent immune responses, while SM cells originate from T-dependent responses. Alternatively, MM cells may be early emigrants from T-dependent germinal centers. Double negative B cells (CD27-IgD-, DN) have been said to be exhausted memory cells, but their precise origin is unclear. Therefore, a definitive elucidation of lineage relationships between these different B cell subsets is needed.

Original language	English
Title of host publication	International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation
Subtitle of host publication	Immunology without Borders, Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781509002986
DOIs	https://doi.org/10.1109/AISW.2015.7469231
State	Published - 11 May 2016
Event	International Workshop on Artificial Immune Systems, AIS 2015 - Taormina, Sicily, Italy Duration: 17 Jul 2015 → 18 Jul 2015

Publication series

Name	International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings

Conference

Conference	International Workshop on Artificial Immune Systems, AIS 2015
Country/Territory	Italy
City	Taormina, Sicily
Period	17/07/15 → 18/07/15

Bibliographical note

Publisher Copyright:
© 2015 IEEE.

Keywords

high-throughput sequencing
immunoglobulin
memory B cells
somatic hypermutation

Access to Document

10.1109/AISW.2015.7469231

Cite this

Hazanov, L., Mehr, R., Wu, Y. C. B., & Dunn-Walters, D. K. (2016). Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways. In International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings Article 7469231 (International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/AISW.2015.7469231

Hazanov, Lena ; Mehr, Ramit ; Wu, Yu Chang Bryan et al. / Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways. International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings. Institute of Electrical and Electronics Engineers Inc., 2016. (International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings).

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keywords = "high-throughput sequencing, immunoglobulin, memory B cells, somatic hypermutation",

author = "Lena Hazanov and Ramit Mehr and Wu, {Yu Chang Bryan} and Dunn-Walters, {Deborah K.}",

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doi = "10.1109/AISW.2015.7469231",

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Hazanov, L, Mehr, R, Wu, YCB & Dunn-Walters, DK 2016, Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways. in International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings., 7469231, International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings, Institute of Electrical and Electronics Engineers Inc., International Workshop on Artificial Immune Systems, AIS 2015, Taormina, Sicily, Italy, 17/07/15. https://doi.org/10.1109/AISW.2015.7469231

Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways. / Hazanov, Lena; Mehr, Ramit; Wu, Yu Chang Bryan et al.
International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings. Institute of Electrical and Electronics Engineers Inc., 2016. 7469231 (International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Hazanov, Lena

AU - Mehr, Ramit

AU - Wu, Yu Chang Bryan

AU - Dunn-Walters, Deborah K.

PY - 2016/5/11

Y1 - 2016/5/11

N2 - Transitional (TR) B cells are immature B cells that have migrated from the bone marrow to peripheral lymphoid organs, but can still undergo selection against autoreactivity. TR cells that survive selection eventually develop into mature naïve B cells (CD27-IgD+, NA). Upon exposure to antigen, NA cells may become IgM memory (CD27+IgD+, MM) or "classical", classswitched memory (CD27+IgD-, SM) B cells. Although MM immunoglobulin (Ig) genes do not undergo class switching, they do undergo somatic hypermutation, albeit with lower frequency than SM. It has been postulated that MM B cells originate from T-independent immune responses, while SM cells originate from T-dependent responses. Alternatively, MM cells may be early emigrants from T-dependent germinal centers. Double negative B cells (CD27-IgD-, DN) have been said to be exhausted memory cells, but their precise origin is unclear. Therefore, a definitive elucidation of lineage relationships between these different B cell subsets is needed.

AB - Transitional (TR) B cells are immature B cells that have migrated from the bone marrow to peripheral lymphoid organs, but can still undergo selection against autoreactivity. TR cells that survive selection eventually develop into mature naïve B cells (CD27-IgD+, NA). Upon exposure to antigen, NA cells may become IgM memory (CD27+IgD+, MM) or "classical", classswitched memory (CD27+IgD-, SM) B cells. Although MM immunoglobulin (Ig) genes do not undergo class switching, they do undergo somatic hypermutation, albeit with lower frequency than SM. It has been postulated that MM B cells originate from T-independent immune responses, while SM cells originate from T-dependent responses. Alternatively, MM cells may be early emigrants from T-dependent germinal centers. Double negative B cells (CD27-IgD-, DN) have been said to be exhausted memory cells, but their precise origin is unclear. Therefore, a definitive elucidation of lineage relationships between these different B cell subsets is needed.

KW - high-throughput sequencing

KW - immunoglobulin

KW - memory B cells

KW - somatic hypermutation

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BT - International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - International Workshop on Artificial Immune Systems, AIS 2015

Y2 - 17 July 2015 through 18 July 2015

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Hazanov L, Mehr R, Wu YCB, Dunn-Walters DK. Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways. In International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings. Institute of Electrical and Electronics Engineers Inc. 2016. 7469231. (International Workshop on Artificial Immune Systems, AIS 2015/ICSI3 2015 - Systems Immunology, Immunoinformatics and Immune-computation: Immunology without Borders, Proceedings). doi: 10.1109/AISW.2015.7469231

Lineage tree analysis of high throughput immunoglobulin sequencing clarifies B cell maturation pathways

Abstract

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Bibliographical note

Keywords

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