Lhx3, a LIM domain transcription factor, is regulated by Pou4f3 in the auditory but not in the vestibular system

Ronna Hertzano, Amiel A. Dror, Mireille Montcouquiol, Zubair M. Ahmed, Buffy Ellsworth, Sally Camper, Thomas B. Friedman, Matthew W. Kelley, Karen B. Avraham

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

A dominant mutation of the gene encoding the POU4F3 transcription factor underlies human non-syndromic progressive hearing loss DFNA15. Using oligonucleotide microarrays to generate expression profiles of inner ears of Pou4f3ddl/ddl mutant and wild-type mice, we have identified and validated Lhx3, a LIM domain transcription factor, as an in vivo target gene regulated by Pou4f3. Lhx3 is a hair cell-specific gene expressed in all hair cells of the auditory and vestibular system as early as embryonic day 16. The level of Lhx3 mRNA is greatly reduced in the inner ears of embryonic Pou4f3 mutant mice. Our data also show that the expression of Lhx3 is regulated differently in auditory and vestibular hair cells. This is the first example of a hair cell-specific gene expressed both in auditory and in vestibular hair cells, with differential regulation of expression in these two closely related systems.

Original languageEnglish
Pages (from-to)999-1005
Number of pages7
JournalEuropean Journal of Neuroscience
Volume25
Issue number4
DOIs
StatePublished - Feb 2007
Externally publishedYes

Keywords

  • Cochlea
  • Deafness
  • Gfi1
  • Hair cell
  • Inner ear
  • Mouse

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