Levels of endogenous cytokinins, indole-3-acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY-2 cells

Pascale Redig, Orit Shaul, Dirk Inzé, Marc Van Montagu, Harry Van Onckelen

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131 Scopus citations

Abstract

Correlation between cell cycle progression and endogenous levels of plant hormones was studied in synchronized tobacco BY-2 cell suspension cultures. Sixteen different cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were extracted using solid-phase anion exchange chromatography in combination with immunoaffinity purification, and quantified by mass spectrometry. No significant correlation could be identified for IAA and ABA. In contrast, there were sharp peaks in the levels of specific cytokinins (zeatin- and dihydrozeatin-type) at the end of the S phase and during mitosis. The levels of other cytokinins analyzed, including zeatins N- and O-glucosides, remained low, suggesting that the increased amounts of their corresponding non-glucosylated form resulted from de novo synthesis. These findings suggest that zeatin- and dihydrozeatin-type cytokinins might play a specific regulatory role in the progression of the plant cell cycle. One hypothesis to explain cytokinin action is based on a specific interaction with kinases that regulate cell cycle progression, as has been recently shown for the cytokinin analogue olomoucine.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalFEBS Letters
Volume391
Issue number1-2
DOIs
StatePublished - 5 Aug 1996
Externally publishedYes

Bibliographical note

Funding Information:
AcknowledgementTsh: e authorsw ish to thankN . Schrijversfo r expert technicaal ssistancae ndW. Van Dongenf or LC-MS/MS analyses.T his work was supportedb y grantsf rom the Belgian Pro-grammeo n InteruniversitPyo les of Attraction( Prime Minister's Office,S cienceP olicyP rogrammin#g,3 8),t heF ondsv oorKollektief FundamenteOenl derzoek(G .0121.96)t,h e VlaamsA ctieprogramma Biotechnologi(eE TC 002),a ndthe K6rberS tiftungO. .S. is indebted to the RothschildF oundatioann dthe EuropeanM olecularB iology Organizatiofno r fellowshipsP. .R. and H.V.O. are a ResearchA ssis-tant and a ResearchD irectoro f the NationalF und for Scientific Research(B elgium)r, espectivelyD.. I. is a ResearchD irectoro f the InstitutN ationadl e la RechercheA gronomiqu(eF rance).

Keywords

  • Cell cycle
  • Cytokinin
  • Mass spectrometry
  • Plant hormone
  • Synchronization
  • Tobacco BY-2 cell

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