Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

Lina Abu-Tair; Sarit Doron; Mahmud Mahamid; Johnny Amer; Rifaat Safadi

doi:10.1016/j.mito.2012.10.012

Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

Lina Abu-Tair, Sarit Doron, Mahmud Mahamid, Johnny Amer, Rifaat Safadi

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.

Original language	English
Pages (from-to)	473-480
Number of pages	8
Journal	Mitochondrion
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/j.mito.2012.10.012
State	Published - Sep 2013
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by the Israel Science Foundation no. 1169/09 . This study was also supported (in part) by grant no. 3-5937 from the Chief Scientist Office of the Ministry of Health, Israel and by the Israel Science Foundation . The authors give special thanks to Dr. Carmel Kasher for his help in the scientific background.

Funding

This research was supported by the Israel Science Foundation no. 1169/09 . This study was also supported (in part) by grant no. 3-5937 from the Chief Scientist Office of the Ministry of Health, Israel and by the Israel Science Foundation . The authors give special thanks to Dr. Carmel Kasher for his help in the scientific background.

Funders	Funder number
Israel Science Foundation	1169/09, 3-5937
Ministry of Health, State of Israel

Keywords

Adherence
Hepatic fibrosis
Leptin receptor
Lymphocytes
Oxidative stress

Access to Document

10.1016/j.mito.2012.10.012

Cite this

@article{77264df31230463a938011c2d3c725d1,

title = "Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations",

abstract = "We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.",

keywords = "Adherence, Hepatic fibrosis, Leptin receptor, Lymphocytes, Oxidative stress",

author = "Lina Abu-Tair and Sarit Doron and Mahmud Mahamid and Johnny Amer and Rifaat Safadi",

note = "Funding Information: This research was supported by the Israel Science Foundation no. 1169/09 . This study was also supported (in part) by grant no. 3-5937 from the Chief Scientist Office of the Ministry of Health, Israel and by the Israel Science Foundation . The authors give special thanks to Dr. Carmel Kasher for his help in the scientific background.",

year = "2013",

month = sep,

doi = "10.1016/j.mito.2012.10.012",

language = "אנגלית",

volume = "13",

pages = "473--480",

journal = "Mitochondrion",

issn = "1567-7249",

publisher = "Elsevier",

number = "5",

}

TY - JOUR

T1 - Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

AU - Abu-Tair, Lina

AU - Doron, Sarit

AU - Mahamid, Mahmud

AU - Amer, Johnny

AU - Safadi, Rifaat

N1 - Funding Information: This research was supported by the Israel Science Foundation no. 1169/09 . This study was also supported (in part) by grant no. 3-5937 from the Chief Scientist Office of the Ministry of Health, Israel and by the Israel Science Foundation . The authors give special thanks to Dr. Carmel Kasher for his help in the scientific background.

PY - 2013/9

Y1 - 2013/9

N2 - We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.

AB - We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.

KW - Adherence

KW - Hepatic fibrosis

KW - Leptin receptor

KW - Lymphocytes

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=84882848649&partnerID=8YFLogxK

U2 - 10.1016/j.mito.2012.10.012

DO - 10.1016/j.mito.2012.10.012

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 23092931

AN - SCOPUS:84882848649

SN - 1567-7249

VL - 13

SP - 473

EP - 480

JO - Mitochondrion

JF - Mitochondrion

IS - 5

ER -

Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this