LEDs for fluorescence microscopy

I. T. Young, Y. Garini, H. R.C. Dietrich, W. Van Oel, G. Liqui Lung

Research output: Contribution to journalConference articlepeer-review

7 Scopus citations

Abstract

Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light source, and possible distortions due to coherence effects. We are examining the possibility of using the new high-power light-emitting diode (LED) sources as alternatives to the above mentioned sources. LED sources are near monochromatic, are inexpensive, produce little heat, have no coherence problems, have extended lifetimes, are small, and can easily be modulated. We describe experiments comparing various LEDs to other light sources. We compare, for example, a 530 nm LED to the 546 nm line from a mercury lamp on a fluorophore whose absorption maximum is broad and in the middle between these two wavelengths.

Original languageEnglish
Article number40
Pages (from-to)208-215
Number of pages8
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5
Issue number13
DOIs
StatePublished - 2004
Externally publishedYes
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI - San Jose, CA, United States
Duration: 27 Jan 200429 Jan 2004

Keywords

  • Fluorescence microscopy
  • High-power light-emitting diodes
  • Multi-spectral sources
  • Quantitative microscopy

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