Laser based enhancement of susceptibility of bacteria to antibiotic

Yana Reznick, Ehud Banin, Anat Lipovsky, Rachel Lubart, Zeev Zalevsky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


Our objective is to test the effect of pulsed (Q-switched) and continuous wave (CW) laser light at wavelength of 532nm on the viability of free-living stationary phase bacteria with and without gentamicin (an antibiotic) treatment. Free living stationary phase gram negative bacteria (Pseudomonas aeruginosa strain PAO1) was immersed in Luria Broth (LB) solution and exposed to Q-switched and CW lasers with and without the addition of the antibiotic gentamicin. Cell viability was determined at different time points. Laser treatment alone did not reduce cell viability compared to untreated control and the gentamicin treatment alone only resulted in a 0.5 log reduction in the viable count for P. aeruginosa. The combined laser and gentamicin treatment, however, resulted in a synergistic effect and viability was reduced by 8 log's for P. aeruginosa PAO1.

Original languageEnglish
Title of host publicationBiophotonics and Immune Responses VII
StatePublished - 2012
EventBiophotonics and Immune Responses VII - San Francisco, CA, United States
Duration: 23 Jan 201223 Jan 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceBiophotonics and Immune Responses VII
Country/TerritoryUnited States
CitySan Francisco, CA


  • Antimicrobial treatment
  • Bacterial viability
  • Gentamicin
  • Laser
  • Medical optics
  • Photodynamic therapy


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