Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon

Dino Marcus, Arye Lazar, Shaul Reuveny, Abraham Traub, Avshalom Mizrahi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal) columns. The IFN specific activity is increased from 1 × 105 units/mg protein, in the crude solution, to 2 × 107 and 2 × 108 units/mg protein, in the purified preparations obtained after polyclonal or monoclonal antibody columns, respectively. The overall recovery of IFN in the procedure described is 50-55%.

Original languageEnglish
Pages (from-to)453-456
Number of pages4
JournalBiotechnology Letters
Volume5
Issue number7
DOIs
StatePublished - Jul 1983
Externally publishedYes

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