Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon

Dino Marcus; Arye Lazar; Shaul Reuveny; Abraham Traub; Avshalom Mizrahi

doi:10.1007/bf00132227

Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon

Dino Marcus, Arye Lazar, Shaul Reuveny, Abraham Traub, Avshalom Mizrahi

Israel Institute for Biological Research

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal) columns. The IFN specific activity is increased from 1 × 10⁵ units/mg protein, in the crude solution, to 2 × 10⁷ and 2 × 10⁸ units/mg protein, in the purified preparations obtained after polyclonal or monoclonal antibody columns, respectively. The overall recovery of IFN in the procedure described is 50-55%.

Original language	English
Pages (from-to)	453-456
Number of pages	4
Journal	Biotechnology Letters
Volume	5
Issue number	7
DOIs	https://doi.org/10.1007/bf00132227
State	Published - Jul 1983
Externally published	Yes

Access to Document

10.1007/bf00132227

Cite this

@article{255efdb580884d23805fbade5d0017a7,

title = "Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon",

abstract = "A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal) columns. The IFN specific activity is increased from 1 × 105 units/mg protein, in the crude solution, to 2 × 107 and 2 × 108 units/mg protein, in the purified preparations obtained after polyclonal or monoclonal antibody columns, respectively. The overall recovery of IFN in the procedure described is 50-55%.",

author = "Dino Marcus and Arye Lazar and Shaul Reuveny and Abraham Traub and Avshalom Mizrahi",

year = "1983",

month = jul,

doi = "10.1007/bf00132227",

language = "אנגלית",

volume = "5",

pages = "453--456",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "7",

}

TY - JOUR

T1 - Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon

AU - Marcus, Dino

AU - Lazar, Arye

AU - Reuveny, Shaul

AU - Traub, Abraham

AU - Mizrahi, Avshalom

PY - 1983/7

Y1 - 1983/7

N2 - A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal) columns. The IFN specific activity is increased from 1 × 105 units/mg protein, in the crude solution, to 2 × 107 and 2 × 108 units/mg protein, in the purified preparations obtained after polyclonal or monoclonal antibody columns, respectively. The overall recovery of IFN in the procedure described is 50-55%.

AB - A procedure for large scale purification of human lymphoblastoid (Namalva) interferon (IFN) is presented. The procedure includes concentration by ultrafiltration, gel filtration on Sephadex G-75 and affinity chromatography on anti-INF antibody (polyclonal or monoclonal) columns. The IFN specific activity is increased from 1 × 105 units/mg protein, in the crude solution, to 2 × 107 and 2 × 108 units/mg protein, in the purified preparations obtained after polyclonal or monoclonal antibody columns, respectively. The overall recovery of IFN in the procedure described is 50-55%.

UR - http://www.scopus.com/inward/record.url?scp=0020964504&partnerID=8YFLogxK

U2 - 10.1007/bf00132227

DO - 10.1007/bf00132227

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0020964504

SN - 0141-5492

VL - 5

SP - 453

EP - 456

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 7

ER -

Large scale production of human lymphoblastoid (Namalva) interferon II. Purification of interferon

Abstract

Access to Document

Other files and links

Fingerprint

Cite this