Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

Amit Tzur; Meirav Noach; Hadas Nevenzal; Yair Glick; Evelin Chorni; Dorit Avrahami; Efrat Barbiro-Michaely; Doron Gerber

doi:10.1096/fasebj.29.1_supplement.884.38

Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

Amit Tzur
, Meirav Noach
, Hadas Nevenzal
, Yair Glick
, Evelin Chorni
, Dorit Avrahami
, Efrat Barbiro-Michaely
, Doron Gerber

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.

Original language	English
Pages (from-to)	884.38
Journal	FASEB Journal
Volume	29
Issue number	S1
DOIs	https://doi.org/10.1096/fasebj.29.1_supplement.884.38
State	Published - 1 Apr 2015

Bibliographical note

https://doi.org/10.1096/fasebj.29.1_supplement.884.38

Access to Document

10.1096/fasebj.29.1_supplement.884.38

Cite this

@article{a82fc4e0a02342eb86a8ea02a704a78e,

title = "Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments",

abstract = "We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.",

author = "Amit Tzur and Meirav Noach and Hadas Nevenzal and Yair Glick and Evelin Chorni and Dorit Avrahami and Efrat Barbiro-Michaely and Doron Gerber",

note = "https://doi.org/10.1096/fasebj.29.1\_supplement.884.38",

year = "2015",

month = apr,

day = "1",

doi = "10.1096/fasebj.29.1\_supplement.884.38",

language = "אנגלית",

volume = "29",

pages = "884.38",

journal = "FASEB Journal",

issn = "0892-6638",

publisher = "John Wiley \& Sons Inc.",

number = "S1",

}

TY - JOUR

T1 - Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

AU - Tzur, Amit

AU - Noach, Meirav

AU - Nevenzal, Hadas

AU - Glick, Yair

AU - Chorni, Evelin

AU - Avrahami, Dorit

AU - Barbiro-Michaely, Efrat

AU - Gerber, Doron

N1 - https://doi.org/10.1096/fasebj.29.1_supplement.884.38

PY - 2015/4/1

Y1 - 2015/4/1

N2 - We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.

AB - We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.

UR - https://www.mendeley.com/catalogue/5c62cff9-caaa-3640-8d64-4a018357dbca/

U2 - 10.1096/fasebj.29.1_supplement.884.38

DO - 10.1096/fasebj.29.1_supplement.884.38

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

SN - 0892-6638

VL - 29

SP - 884.38

JO - FASEB Journal

JF - FASEB Journal

IS - S1

ER -

Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this