Large-scale integrated microfluidics for protein modification discovery in quasi-cellular environments

Amit Tzur, Meirav Noach, Hadas Nevenzal, Yair Glick, Evelin Chorni, Dorit Avrahami, Efrat Barbiro-Michaely, Doron Gerber

Research output: Contribution to journalArticlepeer-review

Abstract

We introduce a modular microfluidic platform for monitoring multiple post-translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi-cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost-effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.
Original languageEnglish
Pages (from-to)884.38
JournalFASEB Journal
Volume29
Issue numberS1
DOIs
StatePublished - 1 Apr 2015

Bibliographical note

https://doi.org/10.1096/fasebj.29.1_supplement.884.38

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