Large-field lattice structured illumination microscopy

Juanjuan Zheng; Xiang Fang; Kai Wen; Jiaoyue Li; Ying Ma; Min Liu; Sha An; Jianlang Li; Zeev Zalevsky; Peng Gao

doi:10.1364/OE.461615

Large-field lattice structured illumination microscopy

Juanjuan Zheng, Xiang Fang, Kai Wen, Jiaoyue Li, Ying Ma, Min Liu, Sha An, Jianlang Li, Zeev Zalevsky, Peng Gao

Bar-Ilan University - The Alexander Kofkin Faculty of Engineering

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMDbased SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.

Original language	English
Pages (from-to)	27951-27966
Number of pages	16
Journal	Optics Express
Volume	30
Issue number	15
DOIs	https://doi.org/10.1364/OE.461615
State	Published - 18 Jul 2022

Bibliographical note

Publisher Copyright:
© 2022 Optica Publishing Group.

Funding

Funding. National Natural Science Foundation of China (62075177, 12104354, 62105251); National Key Research and Development Program of China (2021YFF0700300, 2022YFE0100700); Ministry of Science, Technology and Space (3-18137); International Cooperation and Exchange Programme (2021-2022); China Scholarship Council; Fundamental Research Funds for the Central Universities (QTZX22039, XJS210504); Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermantation, China National Light Industry (2019JJ012); State Key Laboratory of Transient Optics and Photonics.

Funders	Funder number
Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermantation, China National Light Industry	2019JJ012
Ministry of Science, Technology and Space	3-18137
National Natural Science Foundation of China	62075177, 12104354, 62105251
China Scholarship Council
International Cooperation and Exchange Programme	2021-2022
State Key Laboratory of Transient Optics and Photonics
National Key Research and Development Program of China	2021YFF0700300, 2022YFE0100700
Fundamental Research Funds for the Central Universities	XJS210504, QTZX22039

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title = "Large-field lattice structured illumination microscopy",

abstract = "In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMDbased SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.",

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AU - Zheng, Juanjuan

AU - Fang, Xiang

AU - Wen, Kai

AU - Li, Jiaoyue

AU - Ma, Ying

AU - Liu, Min

AU - An, Sha

AU - Li, Jianlang

AU - Zalevsky, Zeev

AU - Gao, Peng

PY - 2022/7/18

Y1 - 2022/7/18

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AB - In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMDbased SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.

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Large-field lattice structured illumination microscopy

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