Abstract
In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMDbased SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.
| Original language | English |
|---|---|
| Pages (from-to) | 27951-27966 |
| Number of pages | 16 |
| Journal | Optics Express |
| Volume | 30 |
| Issue number | 15 |
| DOIs | |
| State | Published - 18 Jul 2022 |
Bibliographical note
Funding Information:Funding. National Natural Science Foundation of China (62075177, 12104354, 62105251); National Key Research and Development Program of China (2021YFF0700300, 2022YFE0100700); Ministry of Science, Technology and Space (3-18137); International Cooperation and Exchange Programme (2021-2022); China Scholarship Council; Fundamental Research Funds for the Central Universities (QTZX22039, XJS210504); Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermantation, China National Light Industry (2019JJ012); State Key Laboratory of Transient Optics and Photonics.
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