Large-field lattice structured illumination microscopy

Juanjuan Zheng, Xiang Fang, Kai Wen, Jiaoyue Li, Ying Ma, Min Liu, Sha An, Jianlang Li, Zeev Zalevsky, Peng Gao

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMDbased SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.

Original languageEnglish
Pages (from-to)27951-27966
Number of pages16
JournalOptics Express
Volume30
Issue number15
DOIs
StatePublished - 18 Jul 2022

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