Laminin 521 modulates the sytotoxic effect of 5-fluorouracil on colorectal cancer ht29 cells

M. P. Raigorodskaya; A. Turchinovich; I. M. Tsypina; V. G. Zgoda; S. V. Nikulin; D. V. Maltseva

doi:10.21519/0234-2758-2019-35-6-73-79

Laminin 521 modulates the sytotoxic effect of 5-fluorouracil on colorectal cancer ht29 cells

M. P. Raigorodskaya, A. Turchinovich, I. M. Tsypina, V. G. Zgoda, S. V. Nikulin, D. V. Maltseva

Research output: Contribution to journal › Article › peer-review

Abstract

The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3 σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription.

Original language	English
Pages (from-to)	73-79
Number of pages	7
Journal	Biotekhnologiya
Volume	35
Issue number	6
DOIs	https://doi.org/10.21519/0234-2758-2019-35-6-73-79
State	Published - 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© State Research Institute for Genetics and Selection of Industrial Microorganisms.

Keywords

14-3-3 σ
5-fluorouracil
Apoptosis
Colorectal cancer
HT29
ITGB1
LAMA5
Laminin 521 (laminin-11)
Regorafenib
SFN
YAP
ß1 integrin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21519/0234-2758-2019-35-6-73-79

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title = "Laminin 521 modulates the sytotoxic effect of 5-fluorouracil on colorectal cancer ht29 cells",

abstract = "The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3 σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription.",

keywords = "14-3-3 σ, 5-fluorouracil, Apoptosis, Colorectal cancer, HT29, ITGB1, LAMA5, Laminin 521 (laminin-11), Regorafenib, SFN, YAP, {\ss}1 integrin",

author = "Raigorodskaya, {M. P.} and A. Turchinovich and Tsypina, {I. M.} and Zgoda, {V. G.} and Nikulin, {S. V.} and Maltseva, {D. V.}",

note = "Publisher Copyright: {\textcopyright} State Research Institute for Genetics and Selection of Industrial Microorganisms.",

year = "2019",

doi = "10.21519/0234-2758-2019-35-6-73-79",

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T1 - Laminin 521 modulates the sytotoxic effect of 5-fluorouracil on colorectal cancer ht29 cells

AU - Raigorodskaya, M. P.

AU - Turchinovich, A.

AU - Tsypina, I. M.

AU - Zgoda, V. G.

AU - Nikulin, S. V.

AU - Maltseva, D. V.

N1 - Publisher Copyright: © State Research Institute for Genetics and Selection of Industrial Microorganisms.

PY - 2019

Y1 - 2019

N2 - The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3 σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription.

AB - The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3 σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription.

KW - 14-3-3 σ

KW - 5-fluorouracil

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KW - Colorectal cancer

KW - HT29

KW - ITGB1

KW - LAMA5

KW - Laminin 521 (laminin-11)

KW - Regorafenib

KW - SFN

KW - YAP

KW - ß1 integrin

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Laminin 521 modulates the sytotoxic effect of 5-fluorouracil on colorectal cancer ht29 cells

Abstract

Bibliographical note

Keywords

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