Laminin 521 Modulates the Сytotoxic Effect of 5-Fluorouracil on HT29 Colorectal Cancer Cells

M. P. Raigorodskaya, A. Turchinovich, I. M. Tsypina, V. G. Zgoda, S. V. Nikulin, D. V. Maltseva

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Abstract: The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF), drugs with different mechanisms of action used to treat colorectal cancer, on an HT29 cell line cultured on plastic or laminin 521 (LM-521) has been studied. It is first shown that LM-521 can increase the sensitivity of tumor cells to 5FU. A possible mechanism of the observed effect of LM-521 on the HT29 cell viability is proposed based on transcriptome and proteome analysis. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways and promote phosphorylation of the YAP transcription coactivator and its binding to the complex with the 14-3-3σ protein. The formation of this complex leads to YAP retention in the cytoplasm and prevents its transport to the nucleus and the activation of antiapoptotic gene transcription.

Original languageEnglish
Pages (from-to)870-874
Number of pages5
JournalApplied Biochemistry and Microbiology
Issue number8
StatePublished - Dec 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020, Pleiades Publishing, Inc.


The proteome analysis was carried out on the equipment of Human Proteome Center for Common Use (IBMKh).

FundersFunder number
Human Proteome Center for Common Use


    • 14-3-3σ
    • 5-fluorouracil
    • HT29
    • ITGB1
    • LAMA5
    • SFN
    • YAP
    • apoptosis
    • colorectal cancer
    • laminin 521 (laminin-11)
    • regorafenib
    • β1 integrin


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