TY - JOUR
T1 - Lack of HER-2 gene amplification in non-Hodgkin lymphoma using chromogenic in situ hybridisation test
AU - Szvalb, Sergio
AU - Stein, Moshe
AU - Gershuny, Anthony
AU - Gez, Eli
AU - Hadary, Amram
AU - Zidan, Jamal
PY - 2009
Y1 - 2009
N2 - HER-2 is overexpressed in 25% of breast cancers and provides a poor prognostic factor, affecting treatment decisions including administration of trastuzumab. Single reports show a lack of HER-2 in non-Hodgkin lymphomas (NHLs) using immunohistochemical (IHC) test. The present study evaluates HER-2 in NHLs using the chromogenic in situ hybridisation (CISH) test, which is more accurate than IHC, to seek new prognostic factors. The secondary aim of the study is to investigate efficacy of using trastuzumab in the treatment of NHLs in future studies. Fifty eight formalin-fixed, paraffin-embedded tissue specimens presenting various NHLs were examined using CISH test. Sixty six percent of all patients were diagnosed at stages III and IV. Histologically, 30 (52%) were low grade and 28 (48%) were intermediate and high grade. HER-2 was negative in all NHLs. Because there is no HER-2 gene amplification in NHL, HER-2 cannot be used as a prognostic factor and should not play a role in biological targeting therapy in non-Hodgkin lymphoma.
AB - HER-2 is overexpressed in 25% of breast cancers and provides a poor prognostic factor, affecting treatment decisions including administration of trastuzumab. Single reports show a lack of HER-2 in non-Hodgkin lymphomas (NHLs) using immunohistochemical (IHC) test. The present study evaluates HER-2 in NHLs using the chromogenic in situ hybridisation (CISH) test, which is more accurate than IHC, to seek new prognostic factors. The secondary aim of the study is to investigate efficacy of using trastuzumab in the treatment of NHLs in future studies. Fifty eight formalin-fixed, paraffin-embedded tissue specimens presenting various NHLs were examined using CISH test. Sixty six percent of all patients were diagnosed at stages III and IV. Histologically, 30 (52%) were low grade and 28 (48%) were intermediate and high grade. HER-2 was negative in all NHLs. Because there is no HER-2 gene amplification in NHL, HER-2 cannot be used as a prognostic factor and should not play a role in biological targeting therapy in non-Hodgkin lymphoma.
KW - CISH test
KW - HER-2
KW - Non-Hodgkin lymphoma
KW - Prognostic factors
UR - http://www.scopus.com/inward/record.url?scp=68449102961&partnerID=8YFLogxK
U2 - 10.1080/10428190902801820
DO - 10.1080/10428190902801820
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C2 - 19452317
AN - SCOPUS:68449102961
SN - 1042-8194
VL - 50
SP - 736
EP - 740
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 5
ER -
