Labeling cytosolic targets in live cells with blinking probes

Jianmin Xu; Jason Chang; Qi Yan; Thomas Dertinger; Marcel P. Bruchez; Shimon Weiss

doi:10.1021/jz400682m

Labeling cytosolic targets in live cells with blinking probes

Jianmin Xu, Jason Chang, Qi Yan, Thomas Dertinger, Marcel P. Bruchez, Shimon Weiss

Research output: Contribution to journal › Review article › peer-review

23 Scopus citations

Abstract

With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.

Original language	English
Pages (from-to)	2138-2146
Number of pages	9
Journal	Journal of Physical Chemistry Letters
Volume	4
Issue number	13
DOIs	https://doi.org/10.1021/jz400682m
State	Published - 3 Jul 2013
Externally published	Yes

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10.1021/jz400682m

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title = "Labeling cytosolic targets in live cells with blinking probes",

abstract = "With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.",

author = "Jianmin Xu and Jason Chang and Qi Yan and Thomas Dertinger and Bruchez, {Marcel P.} and Shimon Weiss",

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AU - Chang, Jason

AU - Yan, Qi

AU - Dertinger, Thomas

AU - Bruchez, Marcel P.

AU - Weiss, Shimon

PY - 2013/7/3

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AB - With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.

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Labeling cytosolic targets in live cells with blinking probes

Abstract

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