Cell-to-cell communication system involves Exosomes, small, membrane-enveloped nanovesicles. Exosomes are evolving as effective therapeutic tools for different pathologies. These extracellular vesicles can bypass biological barriers such as the blood-brain barrier, and can function as powerful nanocarriers for drugs, proteins and gene therapeutics. However, to promote exosomes' therapy development, especially for brain pathologies, a better understanding of their mechanism of action, trafficking, pharmacokinetics and bio-distribution is needed. In this research, we established a new method for non-invasive in-vivo neuroimaging of mesenchymal stem cell (MSC)-derived exosomes, based on computed tomography (CT) imaging with glucose-coated gold nanoparticle (GNP) labeling. We demonstrated that the exosomes were efficiently and directly labeled with GNPs, via an energy-dependent mechanism. Additionally, we found the optimal parameters for exosome labeling and neuroimaging, wherein 5 nm GNPs enhanced labeling, and intranasal administration produced superior brain accumulation. We applied our technique in a mouse model of focal ischemia. Imaging and tracking of intranasally-administered GNP-labeled exosomes revealed specific accumulation and prolonged presence at the lesion area, up to 24 hrs. We propose that this novel exosome labeling and in-vivo neuroimaging technique can serve as a general platform for brain theranostics.
|Title of host publication||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV|
|Editors||Dan V. Nicolau, Alexander N. Cartwright, Dror Fixler|
|State||Published - 2018|
|Event||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018 - San Francisco, United States|
Duration: 30 Jan 2018 → 31 Jan 2018
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Conference||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018|
|Period||30/01/18 → 31/01/18|
Bibliographical noteFunding Information:
This work was partially supported by the doctoral scholarship granted to O.B. by the Ministry of Science and Technology, Israel.
© 2018 SPIE.
- gold nanoparticles