Label-free optical-resolution photoacoustic endomicroscopy in vivo

Joon Mo Yang; Chiye Li; Ruimin Chen; Bin Rao; Junjie Yao; Cheng Hung Yeh; Amos Danielli; Konstantin Maslov; Qifa Zhou; K. Kirk Shung; Lihong V. Wang

doi:10.1117/12.2080224

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Joon Mo Yang, Chiye Li, Ruimin Chen, Bin Rao, Junjie Yao, Cheng Hung Yeh, Amos Danielli, Konstantin Maslov, Qifa Zhou, K. Kirk Shung, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ∼10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

Original language	English
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2015
Editors	Alexander A. Oraevsky, Lihong V. Wang
Publisher	SPIE
ISBN (Electronic)	9781628414134
DOIs	https://doi.org/10.1117/12.2080224
State	Published - 11 Mar 2015
Externally published	Yes
Event	Photons Plus Ultrasound: Imaging and Sensing 2015 - San Francisco, United States Duration: 8 Feb 2015 → 10 Feb 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9323
ISSN (Print)	1605-7422

Conference

Conference	Photons Plus Ultrasound: Imaging and Sensing 2015
Country/Territory	United States
City	San Francisco
Period	8/02/15 → 10/02/15

Bibliographical note

Publisher Copyright:
© 2015 SPIE.

Keywords

In vivo imaging
Intravital microscopy
Label-free imaging
Optical-resolution
Photoacoustic endomicroscopy
Photoacoustic endoscopy
Photoacoustic microscopy
Rat colorectum

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10.1117/12.2080224

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Yang, J. M., Li, C., Chen, R., Rao, B., Yao, J., Yeh, C. H., Danielli, A., Maslov, K., Zhou, Q., Shung, K. K., & Wang, L. V. (2015). Label-free optical-resolution photoacoustic endomicroscopy in vivo. In A. A. Oraevsky, & L. V. Wang (Eds.), Photons Plus Ultrasound: Imaging and Sensing 2015 Article 932332 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9323). SPIE. https://doi.org/10.1117/12.2080224

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abstract = "Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ∼10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.",

keywords = "In vivo imaging, Intravital microscopy, Label-free imaging, Optical-resolution, Photoacoustic endomicroscopy, Photoacoustic endoscopy, Photoacoustic microscopy, Rat colorectum",

author = "Yang, {Joon Mo} and Chiye Li and Ruimin Chen and Bin Rao and Junjie Yao and Yeh, {Cheng Hung} and Amos Danielli and Konstantin Maslov and Qifa Zhou and Shung, {K. Kirk} and Wang, {Lihong V.}",

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doi = "10.1117/12.2080224",

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Yang, JM, Li, C, Chen, R, Rao, B, Yao, J, Yeh, CH, Danielli, A, Maslov, K, Zhou, Q, Shung, KK & Wang, LV 2015, Label-free optical-resolution photoacoustic endomicroscopy in vivo. in AA Oraevsky & LV Wang (eds), Photons Plus Ultrasound: Imaging and Sensing 2015., 932332, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9323, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2015, San Francisco, United States, 8/02/15. https://doi.org/10.1117/12.2080224

Label-free optical-resolution photoacoustic endomicroscopy in vivo. / Yang, Joon Mo; Li, Chiye; Chen, Ruimin et al.
Photons Plus Ultrasound: Imaging and Sensing 2015. ed. / Alexander A. Oraevsky; Lihong V. Wang. SPIE, 2015. 932332 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9323).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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ER -

Label-free optical-resolution photoacoustic endomicroscopy in vivo

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Keywords

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