Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy

Diana Maltseva; Maria Raygorodskaya; Evgeny Knyazev; Victor Zgoda; Olga Tikhonova; Shan Zaidi; Sergey Nikulin; Ancha Baranova; Andrey Turchinovich; Sergey Rodin; Alexander Tonevitsky

doi:10.1016/j.biochi.2020.04.016

Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy

Diana Maltseva
, Maria Raygorodskaya
, Evgeny Knyazev
, Victor Zgoda
, Olga Tikhonova
, Shan Zaidi
, Sergey Nikulin
, Ancha Baranova
, Andrey Turchinovich
, Sergey Rodin
, Alexander Tonevitsky

Higher School of Economics
Russian Academy of Sciences
Bioclinicum Scientific Research Center
Russian Academy of Medical Sciences - Institute of Biomedical Chemistry
College of Science
Moscow Institute of Physics and Technology
Research Centre for Medical Genetics
SciBerg e.Kfm
Uppsala University

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we demonstrate that shRNA-mediated knockdown of the α5 laminin chain results in Wnt- and mTORC1-dependent partial dedifferentiation of colorectal cancer cells. Furthermore, we showed that this dedifferentiation involved activation of ER-stress signaling, pathway promoting the sensitivity of cells to 5-fluorouracil.

Original language	English
Pages (from-to)	107-116
Number of pages	10
Journal	Biochimie
Volume	174
DOIs	https://doi.org/10.1016/j.biochi.2020.04.016
State	Published - Jul 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)

Funding

The study was supported by the Russian Science Foundation (project 17-14-01338 ).

Funders	Funder number
Russian Science Foundation	17-14-01338

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Colorectal cancer
Dedifferentiation
HT29
Intestinal epithelium stem cell marker
LAMA5
Laminin

Access to Document

10.1016/j.biochi.2020.04.016

Cite this

@article{5a8f7d2f23e848b1bb0f54743079bff3,

title = "Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy",

abstract = "The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we demonstrate that shRNA-mediated knockdown of the α5 laminin chain results in Wnt- and mTORC1-dependent partial dedifferentiation of colorectal cancer cells. Furthermore, we showed that this dedifferentiation involved activation of ER-stress signaling, pathway promoting the sensitivity of cells to 5-fluorouracil.",

keywords = "Colorectal cancer, Dedifferentiation, HT29, Intestinal epithelium stem cell marker, LAMA5, Laminin",

author = "Diana Maltseva and Maria Raygorodskaya and Evgeny Knyazev and Victor Zgoda and Olga Tikhonova and Shan Zaidi and Sergey Nikulin and Ancha Baranova and Andrey Turchinovich and Sergey Rodin and Alexander Tonevitsky",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. and Soci{\'e}t{\'e} Fran{\c c}aise de Biochimie et Biologie Mol{\'e}culaire (SFBBM)",

year = "2020",

month = jul,

doi = "10.1016/j.biochi.2020.04.016",

language = "אנגלית",

volume = "174",

pages = "107--116",

journal = "Biochimie",

issn = "0300-9084",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy

AU - Maltseva, Diana

AU - Raygorodskaya, Maria

AU - Knyazev, Evgeny

AU - Zgoda, Victor

AU - Tikhonova, Olga

AU - Zaidi, Shan

AU - Nikulin, Sergey

AU - Baranova, Ancha

AU - Turchinovich, Andrey

AU - Rodin, Sergey

AU - Tonevitsky, Alexander

PY - 2020/7

Y1 - 2020/7

N2 - The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we demonstrate that shRNA-mediated knockdown of the α5 laminin chain results in Wnt- and mTORC1-dependent partial dedifferentiation of colorectal cancer cells. Furthermore, we showed that this dedifferentiation involved activation of ER-stress signaling, pathway promoting the sensitivity of cells to 5-fluorouracil.

AB - The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we demonstrate that shRNA-mediated knockdown of the α5 laminin chain results in Wnt- and mTORC1-dependent partial dedifferentiation of colorectal cancer cells. Furthermore, we showed that this dedifferentiation involved activation of ER-stress signaling, pathway promoting the sensitivity of cells to 5-fluorouracil.

KW - Colorectal cancer

KW - Dedifferentiation

KW - HT29

KW - Intestinal epithelium stem cell marker

KW - LAMA5

KW - Laminin

UR - https://www.scopus.com/pages/publications/85084082374

U2 - 10.1016/j.biochi.2020.04.016

DO - 10.1016/j.biochi.2020.04.016

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 32334043

AN - SCOPUS:85084082374

SN - 0300-9084

VL - 174

SP - 107

EP - 116

JO - Biochimie

JF - Biochimie

ER -

Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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