Abstract
The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. In this work, we demonstrate that shRNA-mediated knockdown of the α5 laminin chain results in Wnt- and mTORC1-dependent partial dedifferentiation of colorectal cancer cells. Furthermore, we showed that this dedifferentiation involved activation of ER-stress signaling, pathway promoting the sensitivity of cells to 5-fluorouracil.
Original language | English |
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Pages (from-to) | 107-116 |
Number of pages | 10 |
Journal | Biochimie |
Volume | 174 |
DOIs | |
State | Published - Jul 2020 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)
Funding
The study was supported by the Russian Science Foundation (project 17-14-01338 ).
Funders | Funder number |
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Russian Science Foundation | 17-14-01338 |
Keywords
- Colorectal cancer
- Dedifferentiation
- HT29
- Intestinal epithelium stem cell marker
- LAMA5
- Laminin