Karyotype analysis of the monogenetic trypanosomatid Leptomonas collosoma

Oded Béjà; Dov Schwartz; Shulamit Michaeli

doi:10.1016/0166-6851(94)90037-x

Karyotype analysis of the monogenetic trypanosomatid Leptomonas collosoma

Oded Béjà, Dov Schwartz, Shulamit Michaeli

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

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Abstract

In order to develop a genetic system for the monogenetic trypanosomatids, we have analyzed the molecular karyotype of Leptomonas collosoma based on chromosome separation by clamped homogeneous electric field (CHEF) gel electrophoresis. The chromosome location of 5 RNA coding genes (SL, U6, 5S, 7SL and rRNA) and 2 protein coding genes (for HSP83 and α-tubulin) was determined. All of the L. collosoma genes examined were found on at least 2 chromosomes, which differ in size in the range of 100-500 kb, suggesting that the organism is diploid. The weighted sum of L.collosoma chromosomes separated by CHEF analysis was approximately 62 ± 3 Mb, whereas the genome size determined by FACS was estimated at approx. 80 Mb. This suggests that some of the homologous chromosomes differ in their size. The analysis presented here may facilitate studies on the function of individual genes, and on the genetic stability of this organism.

Original language	English
Pages (from-to)	71-81
Number of pages	11
Journal	Molecular and Biochemical Parasitology
Volume	66
Issue number	1
DOIs	https://doi.org/10.1016/0166-6851(94)90037-x
State	Published - Jul 1994
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by a grant from the John D. and Catherine T. MacArthur Foundation to the Weizmann Institute of Science. We wish to thank Drs. Nina Agabian, Lionel Schnur, Joseph Shlomai and Charles L. Jaffe for providing the different trypanosomatid species. To Drs. Nina Agabian, Elis-abetta Ullu, Jeremy C. Mottram, William J. Murphy and Kenneth P. Watkins for the gift of the T. brucei gene probes.

Funding

This work was supported by a grant from the John D. and Catherine T. MacArthur Foundation to the Weizmann Institute of Science. We wish to thank Drs. Nina Agabian, Lionel Schnur, Joseph Shlomai and Charles L. Jaffe for providing the different trypanosomatid species. To Drs. Nina Agabian, Elis-abetta Ullu, Jeremy C. Mottram, William J. Murphy and Kenneth P. Watkins for the gift of the T. brucei gene probes.

Funders	Funder number
John D. and Catherine T. MacArthur Foundation
Weizmann Institute of Science

Keywords

Karyotype
Leishmania
Leptomonas
Trypanosoma

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10.1016/0166-6851(94)90037-x

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title = "Karyotype analysis of the monogenetic trypanosomatid Leptomonas collosoma",

abstract = "In order to develop a genetic system for the monogenetic trypanosomatids, we have analyzed the molecular karyotype of Leptomonas collosoma based on chromosome separation by clamped homogeneous electric field (CHEF) gel electrophoresis. The chromosome location of 5 RNA coding genes (SL, U6, 5S, 7SL and rRNA) and 2 protein coding genes (for HSP83 and α-tubulin) was determined. All of the L. collosoma genes examined were found on at least 2 chromosomes, which differ in size in the range of 100-500 kb, suggesting that the organism is diploid. The weighted sum of L.collosoma chromosomes separated by CHEF analysis was approximately 62 ± 3 Mb, whereas the genome size determined by FACS was estimated at approx. 80 Mb. This suggests that some of the homologous chromosomes differ in their size. The analysis presented here may facilitate studies on the function of individual genes, and on the genetic stability of this organism.",

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N2 - In order to develop a genetic system for the monogenetic trypanosomatids, we have analyzed the molecular karyotype of Leptomonas collosoma based on chromosome separation by clamped homogeneous electric field (CHEF) gel electrophoresis. The chromosome location of 5 RNA coding genes (SL, U6, 5S, 7SL and rRNA) and 2 protein coding genes (for HSP83 and α-tubulin) was determined. All of the L. collosoma genes examined were found on at least 2 chromosomes, which differ in size in the range of 100-500 kb, suggesting that the organism is diploid. The weighted sum of L.collosoma chromosomes separated by CHEF analysis was approximately 62 ± 3 Mb, whereas the genome size determined by FACS was estimated at approx. 80 Mb. This suggests that some of the homologous chromosomes differ in their size. The analysis presented here may facilitate studies on the function of individual genes, and on the genetic stability of this organism.

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Karyotype analysis of the monogenetic trypanosomatid Leptomonas collosoma

Abstract

Bibliographical note

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Keywords

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