Junction formation in trypsinized cells of human adenocarcinoma cell line

Sylvie Polak-Charcon, J. Shoham, Y. Ben-Shaul

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


A human colon adenocarcinoma cell line was used to study junction formation and disassembly. Monolayered cells grown to confluency have desmosomes but no gap or tight junctions. Treatment with trypsin, while causing the breaking up of desmosomes to hemi-desmosomes, resulted in a rapid assembly of junctions. Tight junctions were formed in some recognizable steps: elevation of particle-free membrane 'crests', alignment of particles on these crests, and fusion of these particles to form typical tight junctions ridges. Gap junctions were also formed on particle-free membrane areas, but comparatively few such junctions were formed. Cycloheximide had no effect on the assembly of junctions. It is therefore assumed that pre-existing membrane particles were rearranged into junctions and that this rearrangement is probably due to the increased mobility of the trypsinized cell membranes. Transfer of trypsinized cells back into trypsin-free fresh medium resulted in internalization of phagocytic-like vesicles containing tight junctions elements.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalExperimental Cell Research
Issue number1
StatePublished - 1 Oct 1978
Externally publishedYes


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