TY - JOUR
T1 - Isolation, purification and preliminary X-ray characterization of Cpn60-2 (65 kDa heat-shock protein) from Mycobacterium tuberculosis
AU - Adir, Noam
AU - Dobrovetsky, Elena
AU - Shafat, Itay
AU - Cohen, Cyril
AU - Kashi, Yechezkel
PY - 2002/9
Y1 - 2002/9
N2 - Cpn60-2 is a member of a unique family of putative molecular chaperones homologous to GroEL (Cpn60) but of unknown function and found only in Mycobacterium tuberculosis and closely related species. Cpn60-2 has mainly been studied for its strong immunogenity. Here, the purification, crystallization and preliminary crystallographic analysis of M. tuberculosis Cpn60-2 are reported. The crystals belong to space group P2, with unit-cell parameters a = 57, b = 115.5, c = 81.5 Å, β = 95.5°, and contain a dimer in the asymmetric unit. The crystals diffract to 4.0 Å using a Cu rotating-anode X-ray generator.
AB - Cpn60-2 is a member of a unique family of putative molecular chaperones homologous to GroEL (Cpn60) but of unknown function and found only in Mycobacterium tuberculosis and closely related species. Cpn60-2 has mainly been studied for its strong immunogenity. Here, the purification, crystallization and preliminary crystallographic analysis of M. tuberculosis Cpn60-2 are reported. The crystals belong to space group P2, with unit-cell parameters a = 57, b = 115.5, c = 81.5 Å, β = 95.5°, and contain a dimer in the asymmetric unit. The crystals diffract to 4.0 Å using a Cu rotating-anode X-ray generator.
UR - http://www.scopus.com/inward/record.url?scp=0036712143&partnerID=8YFLogxK
U2 - 10.1107/s0907444902010909
DO - 10.1107/s0907444902010909
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C2 - 12198306
AN - SCOPUS:0036712143
SN - 0907-4449
VL - 58
SP - 1474
EP - 1475
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 9
ER -