Abstract
A method for isolating a mitochondrial fraction from ejaculated ram spermatozoa is described. It includes treatment with 10 mM dithiothreitol at 0°C for 20 mm followed by homogenization, fractionation on a sand column, and differential centrifugation. The isolated fraction contained about 0.3% of the total sperm DNA, showed a fivefold enrichment in cytochrome oxidase and succinate dehydrogenase activities, and twofold enrichment of NADH dehydrogenase activity. The mitochondrial fraction comprised mainly residual mitochondrial helices, and individual, crescent-shaped mitochondria measuring 0.165 μm wide and 1.256 μm long.
| Original language | English |
|---|---|
| Pages (from-to) | 68-76 |
| Number of pages | 9 |
| Journal | Journal of Structural Biology |
| Volume | 57 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1976 |
Bibliographical note
Funding Information:We are grateful to Professor M. Herzberg und Dr. S. Grossman, of Bar-Ilan University, for their cooperation and helpful discussions. We thank Dr. N. Ayalon, Mr. M. Eisenberg, and Mr. Y. Lewis, of the Kimron Veterinary Institute, Belt Dagan, for their assistance during the course of this study. We gratefully acknowledge the contribution of Miss J. Fisher and the excellent technical assistance of Mrs. Z. Goldwitch in chemical analysis, Mrs. V. Harel in electron microscopy and Mr. U. Yoran in photography. This work was supported by the Research Committee of Bar-Ilan University, Research Grant No. 161-83-26-4.
Funding
We are grateful to Professor M. Herzberg und Dr. S. Grossman, of Bar-Ilan University, for their cooperation and helpful discussions. We thank Dr. N. Ayalon, Mr. M. Eisenberg, and Mr. Y. Lewis, of the Kimron Veterinary Institute, Belt Dagan, for their assistance during the course of this study. We gratefully acknowledge the contribution of Miss J. Fisher and the excellent technical assistance of Mrs. Z. Goldwitch in chemical analysis, Mrs. V. Harel in electron microscopy and Mr. U. Yoran in photography. This work was supported by the Research Committee of Bar-Ilan University, Research Grant No. 161-83-26-4.
| Funders | Funder number |
|---|---|
| Research Committee of Bar-Ilan University | 161-83-26-4 |