Isolation and characterization of primary NK cells and the enrichment of the KIR2DL1+ population

Batel Sabag; Abhishek Puthenveetil; Mira Barda-Saad

doi:10.1016/bs.mcb.2023.09.001

Isolation and characterization of primary NK cells and the enrichment of the KIR2DL1⁺ population

Batel Sabag, Abhishek Puthenveetil, Mira Barda-Saad

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Natural killer (NK) cells are cytotoxic innate lymphoid cells that play critical roles in the mitigation of viral infections and cancer through the secretion of cytolytic granules and immunomodulatory cytokines. Abnormalities in NK function can lead to viral infections, autoimmunity, and cancer. The current protocol provides an NK isolation technique to study the signaling pathways downstream to the Killer cell immunoglobulin-like receptors (KIR) that serve as key human NK cell function regulators. This procedure enables investigating mechanisms specific to individual KIRs to improve our understanding of NK cell function in health and disease.

Original language	English
Title of host publication	The Immunological Synapse - Part C
Editors	Clément Thomas, Lorenzo Galluzzi
Publisher	Academic Press Inc.
Pages	201-211
Number of pages	11
ISBN (Print)	9780443218682
DOIs	https://doi.org/10.1016/bs.mcb.2023.09.001
State	Published - Jan 2025

Publication series

Name	Methods in Cell Biology
Volume	193
ISSN (Print)	0091-679X

Bibliographical note

Publisher Copyright:
© 2025 Elsevier Inc.

Funding

This research was funded by the Israel Science Foundation (ISF) grant no. 1001/23.

Funders	Funder number
Israel Science Foundation	1001/23

Keywords

Inhibitory receptors
KIR
KIR2DL1 isolation
NK cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/bs.mcb.2023.09.001

Cite this

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Isolation and characterization of primary NK cells and the enrichment of the KIR2DL1⁺ population. / Sabag, Batel; Puthenveetil, Abhishek; Barda-Saad, Mira.
The Immunological Synapse - Part C. ed. / Clément Thomas; Lorenzo Galluzzi. Academic Press Inc., 2025. p. 201-211 (Methods in Cell Biology; Vol. 193).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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