Isolation and characterization of primary NK cells and the enrichment of the KIR2DL1+ population

Batel Sabag, Abhishek Puthenveetil, Mira Barda-Saad

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Natural killer (NK) cells are cytotoxic innate lymphoid cells that play critical roles in the mitigation of viral infections and cancer through the secretion of cytolytic granules and immunomodulatory cytokines. Abnormalities in NK function can lead to viral infections, autoimmunity, and cancer. The current protocol provides an NK isolation technique to study the signaling pathways downstream to the Killer cell immunoglobulin-like receptors (KIR) that serve as key human NK cell function regulators. This procedure enables investigating mechanisms specific to individual KIRs to improve our understanding of NK cell function in health and disease.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
DOIs
StateAccepted/In press - 2024

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X

Bibliographical note

Publisher Copyright:
© 2024 Elsevier Inc.

Funding

This research was funded by the Israel Science Foundation (ISF) grant no. 1001/23.

FundersFunder number
Israel Science Foundation1001/23

    Keywords

    • Inhibitory receptors
    • KIR
    • KIR2DL1 isolation
    • NK cells

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