Abstract
A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.
Original language | English |
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Pages (from-to) | 9-12 |
Number of pages | 4 |
Journal | FEMS Microbiology Letters |
Volume | 137 |
Issue number | 1 |
DOIs | |
State | Published - 15 Mar 1996 |
Externally published | Yes |
Bibliographical note
Funding Information:We thank Prof. Y. Heimer and Mr. S. Barak for reading the manuscript.T his work was supportedb y the Rashi Foundation and an Eshcol grant from the Israeli Ministry of Science and Technology to A.N.
Keywords
- Nitrifying bacteria
- Nitrosomonas europaea
- Promoter analysis
- Transcription initiation