Isolation and characterization of a functional promoter from Nitrosomonas europaea

A. Nejidat, O. Spector, A. Abeliovich

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A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalFEMS Microbiology Letters
Issue number1
StatePublished - 15 Mar 1996
Externally publishedYes

Bibliographical note

Funding Information:
We thank Prof. Y. Heimer and Mr. S. Barak for reading the manuscript.T his work was supportedb y the Rashi Foundation and an Eshcol grant from the Israeli Ministry of Science and Technology to A.N.


  • Nitrifying bacteria
  • Nitrosomonas europaea
  • Promoter analysis
  • Transcription initiation


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