Abstract
A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.
| Original language | English |
|---|---|
| Pages (from-to) | 9-12 |
| Number of pages | 4 |
| Journal | FEMS Microbiology Letters |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| State | Published - 15 Mar 1996 |
| Externally published | Yes |
Bibliographical note
Funding Information:We thank Prof. Y. Heimer and Mr. S. Barak for reading the manuscript.T his work was supportedb y the Rashi Foundation and an Eshcol grant from the Israeli Ministry of Science and Technology to A.N.
Funding
We thank Prof. Y. Heimer and Mr. S. Barak for reading the manuscript.T his work was supportedb y the Rashi Foundation and an Eshcol grant from the Israeli Ministry of Science and Technology to A.N.
| Funders | Funder number |
|---|---|
| Israeli ministry of science and technology | |
| Rashi Foundation |
Keywords
- Nitrifying bacteria
- Nitrosomonas europaea
- Promoter analysis
- Transcription initiation