Involvement of K+ channels in the relaxant effects of YC-1 in vascular smooth muscle

Sabine Seitz, Jörg W. Wegener, Johanna Rupp, Makino Watanabe, Andreas Jost, Rolf Gerhard, Asher Shainberg, Rikuo Ochi, Hermann Nawrath

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12 Scopus citations


This study addresses the question whether K+ channels are involved in the vasorelaxant effects of 3-(5'-hydroxymethyl-2'-furyl)-1-benzyl-indazole (YC-1). In rat aorta, guinea pig aorta, and guinea pig a. carotis, YC-1 inhibited contractions induced by phenylephrine (3 μM) more potently than those induced by K+(48 mM). In rat aorta, tetraethylammonium (10 mM), charybdotoxin (0.2 μM), and iberiotoxin (0.1 μM), but not glibenclamide (10 μM), attenuated the relaxant effects of YC-1. In guinea pig a. carotis, YC-1 (30 μM) induced a hyperpolarisation which was antagonised by 1H- [1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 50 μM). In rat aorta, YC-1 (30 μM) increased the rate constant of 86Rb-efflux. The effect of YC-1 was potentiated by zaprinast (10 μM), but inhibited by ODQ (50 μM) or charybdotoxin (0.2 μM). In smooth muscle cells from rat aorta, YC-1 (10 μM) increased BK(Ca) channel activity. It is suggested that YC-1-induced vasorelaxation is partially mediated by the activation of K+ channels.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalEuropean Journal of Pharmacology
Issue number1
StatePublished - 1 Oct 1999

Bibliographical note

Funding Information:
We thank Dr. Schönafinger, Hoechst (Germany), for providing us with YC-1. This work was supported by grants (to H.N.) from the Deutsche Forschungsgemeinschaft (Germany) and the Umweltministerium of Rheinland-Pfalz (Germany).


  • BK(Ca) channel
  • Cyclic GMP
  • Guanylyl cyclase, soluble
  • Relaxation
  • Smooth muscle, vascular


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