Investigation on methanol induced cell wall degradation in plants using home-built LSFM system

S. R. Rinsa; Aditya Kurup; Mayanglambam Suheshkumar Singh

doi:10.1117/12.2609997

Investigation on methanol induced cell wall degradation in plants using home-built LSFM system

S. R. Rinsa, Aditya Kurup, Mayanglambam Suheshkumar Singh

Indian Institute of Science Education and Research Thiruvananthapuram

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The cell wall is the hard layer outside the cell membrane, composed of cellulose, and provides strength to the plant body. Moreover, the tensile strength of the cell wall helps in maintaining the mechanical rigidity and expansion of cells. Any deformation to the cell wall will affect the vitality of the plants. Studies reveal the solubility of cellulose in alcohols, and here we report the study of endurance of cell wall when exposed to methanol. We employed a home-built light sheet microscopy system to visualize the time-dependent deterioration of the cell wall due to the intake of methanol by the plant. The studies were repeated for different concentrations of methanol and show a concentration-dependent degradation curve.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX
Editors	Attila Tarnok, Jessica P. Houston
Publisher	SPIE
ISBN (Electronic)	9781510647992
DOIs	https://doi.org/10.1117/12.2609997
State	Published - 2022
Externally published	Yes
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX 2022 - Virtual, Online Duration: 20 Feb 2022 → 24 Feb 2022

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11964
ISSN (Print)	1605-7422

Conference

Conference	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX 2022
City	Virtual, Online
Period	20/02/22 → 24/02/22

Bibliographical note

Publisher Copyright:
© 2022 SPIE. Downloading of the abstract is permitted for personal use only.

Keywords

Allium cepa
Light Sheet Microscopy
cell wall degradation
fluorescence imaging
methanol
optical microscope
sMx-SPIM

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10.1117/12.2609997

Cite this

Rinsa, S. R., Kurup, A., & Singh, M. S. (2022). Investigation on methanol induced cell wall degradation in plants using home-built LSFM system. In A. Tarnok, & J. P. Houston (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX Article 119640A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11964). SPIE. https://doi.org/10.1117/12.2609997

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Rinsa, SR, Kurup, A & Singh, MS 2022, Investigation on methanol induced cell wall degradation in plants using home-built LSFM system. in A Tarnok & JP Houston (eds), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX., 119640A, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11964, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX 2022, Virtual, Online, 20/02/22. https://doi.org/10.1117/12.2609997

Investigation on methanol induced cell wall degradation in plants using home-built LSFM system. / Rinsa, S. R.; Kurup, Aditya; Singh, Mayanglambam Suheshkumar.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX. ed. / Attila Tarnok; Jessica P. Houston. SPIE, 2022. 119640A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11964).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Investigation on methanol induced cell wall degradation in plants using home-built LSFM system

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