Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM

S. R. Rinsa; Bejoy Manoj; Mayanglambam Suheshkumar Singh

doi:10.1117/12.2583163

Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM

S. R. Rinsa, Bejoy Manoj, Mayanglambam Suheshkumar Singh

Indian Institute of Science Education and Research Thiruvananthapuram

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

Being the largest organelle in a cell, the nucleus houses the genetic code of an organism and primarily serves as the cell’s information center. Proper implementation and consolidation of physiological stress are intrinsic for proper growth and development. Studies reveal that in animals, under chronic stress, the nuclear size shows morphological plasticity. This paper proposes the chronic stress study on plant specimens, namely, Allium cepa root, upon induction on Rhodamine B fluorophore, using our in-house developed dual arm multi-level magnification light sheet microscopy (DMx-LSFM) system. Employing our home-built microscope, we can analyze the difference in girth of the nuclear membrane under different magnification levels simultaneously, which helps to inspect the minute details and structural changes occurring in the organelle. The study was then further extended to investigate the role of exposure time and concentration of the stress agent on the sample.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX
Editors	Irene Georgakoudi, Attila Tarnok, James F. Leary
Publisher	SPIE
ISBN (Electronic)	9781510641297
DOIs	https://doi.org/10.1117/12.2583163
State	Published - 2021
Externally published	Yes
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX 2021 - Virtual, Online, United States Duration: 6 Mar 2021 → 11 Mar 2021

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11647
ISSN (Print)	1605-7422

Conference

Conference	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX 2021
Country/Territory	United States
City	Virtual, Online
Period	6/03/21 → 11/03/21

Bibliographical note

Publisher Copyright:
© 2021 SPIE

Keywords

Allium cepa
Chronic stress
Fluorescence imaging
Light Sheet Microscopy
Nuclear structure
Optical microscope
Rhodamine B

Access to Document

10.1117/12.2583163

Cite this

Rinsa, S. R., Manoj, B., & Singh, M. S. (2021). Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM. In I. Georgakoudi, A. Tarnok, & J. F. Leary (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX Article 116470B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11647). SPIE. https://doi.org/10.1117/12.2583163

Rinsa, S. R. ; Manoj, Bejoy ; Singh, Mayanglambam Suheshkumar. / Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX. editor / Irene Georgakoudi ; Attila Tarnok ; James F. Leary. SPIE, 2021. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{979d5fceb10f42719f0861df776e35c6,

title = "Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM",

abstract = "Being the largest organelle in a cell, the nucleus houses the genetic code of an organism and primarily serves as the cell{\textquoteright}s information center. Proper implementation and consolidation of physiological stress are intrinsic for proper growth and development. Studies reveal that in animals, under chronic stress, the nuclear size shows morphological plasticity. This paper proposes the chronic stress study on plant specimens, namely, Allium cepa root, upon induction on Rhodamine B fluorophore, using our in-house developed dual arm multi-level magnification light sheet microscopy (DMx-LSFM) system. Employing our home-built microscope, we can analyze the difference in girth of the nuclear membrane under different magnification levels simultaneously, which helps to inspect the minute details and structural changes occurring in the organelle. The study was then further extended to investigate the role of exposure time and concentration of the stress agent on the sample.",

keywords = "Allium cepa, Chronic stress, Fluorescence imaging, Light Sheet Microscopy, Nuclear structure, Optical microscope, Rhodamine B",

author = "Rinsa, {S. R.} and Bejoy Manoj and Singh, {Mayanglambam Suheshkumar}",

note = "Publisher Copyright: {\textcopyright} 2021 SPIE; Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX 2021 ; Conference date: 06-03-2021 Through 11-03-2021",

year = "2021",

doi = "10.1117/12.2583163",

language = "אנגלית",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Irene Georgakoudi and Attila Tarnok and Leary, {James F.}",

booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX",

address = "ארצות הברית",

}

Rinsa, SR, Manoj, B & Singh, MS 2021, Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM. in I Georgakoudi, A Tarnok & JF Leary (eds), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX., 116470B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11647, SPIE, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX 2021, Virtual, Online, United States, 6/03/21. https://doi.org/10.1117/12.2583163

Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM. / Rinsa, S. R.; Manoj, Bejoy; Singh, Mayanglambam Suheshkumar.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX. ed. / Irene Georgakoudi; Attila Tarnok; James F. Leary. SPIE, 2021. 116470B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11647).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM

AU - Rinsa, S. R.

AU - Manoj, Bejoy

AU - Singh, Mayanglambam Suheshkumar

PY - 2021

Y1 - 2021

N2 - Being the largest organelle in a cell, the nucleus houses the genetic code of an organism and primarily serves as the cell’s information center. Proper implementation and consolidation of physiological stress are intrinsic for proper growth and development. Studies reveal that in animals, under chronic stress, the nuclear size shows morphological plasticity. This paper proposes the chronic stress study on plant specimens, namely, Allium cepa root, upon induction on Rhodamine B fluorophore, using our in-house developed dual arm multi-level magnification light sheet microscopy (DMx-LSFM) system. Employing our home-built microscope, we can analyze the difference in girth of the nuclear membrane under different magnification levels simultaneously, which helps to inspect the minute details and structural changes occurring in the organelle. The study was then further extended to investigate the role of exposure time and concentration of the stress agent on the sample.

AB - Being the largest organelle in a cell, the nucleus houses the genetic code of an organism and primarily serves as the cell’s information center. Proper implementation and consolidation of physiological stress are intrinsic for proper growth and development. Studies reveal that in animals, under chronic stress, the nuclear size shows morphological plasticity. This paper proposes the chronic stress study on plant specimens, namely, Allium cepa root, upon induction on Rhodamine B fluorophore, using our in-house developed dual arm multi-level magnification light sheet microscopy (DMx-LSFM) system. Employing our home-built microscope, we can analyze the difference in girth of the nuclear membrane under different magnification levels simultaneously, which helps to inspect the minute details and structural changes occurring in the organelle. The study was then further extended to investigate the role of exposure time and concentration of the stress agent on the sample.

KW - Allium cepa

KW - Chronic stress

KW - Fluorescence imaging

KW - Light Sheet Microscopy

KW - Nuclear structure

KW - Optical microscope

KW - Rhodamine B

UR - http://www.scopus.com/inward/record.url?scp=85107856837&partnerID=8YFLogxK

U2 - 10.1117/12.2583163

DO - 10.1117/12.2583163

M3 - ???researchoutput.researchoutputtypes.contributiontobookanthology.conference???

AN - SCOPUS:85107856837

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX

A2 - Georgakoudi, Irene

A2 - Tarnok, Attila

A2 - Leary, James F.

PB - SPIE

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX 2021

Y2 - 6 March 2021 through 11 March 2021

ER -

Rinsa SR, Manoj B, Singh MS. Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM. In Georgakoudi I, Tarnok A, Leary JF, editors, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX. SPIE. 2021. 116470B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2583163

Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this