Abstract
Abstract: In the present study, we assessed the role of annexin 13 membrane-binding protein (ANXA13) in the intracellular transport of vesicles containing type II ribosome-inactivating proteins (RIP-IIs). A modified human intestinal epithelial cell line HT29 was used, in which the expression of ANXA13 was significantly reduced. The cytotoxic effect of ricin and viscumin was evaluated by modification of 28S ribosome RNA. The observed differences in the activity of toxins on the parental and modified HT29 lines indicate that ANXA13 plays a different role in the intracellular transport of vesicles containing the RIP-IIs.
Original language | English |
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Pages (from-to) | 219-221 |
Number of pages | 3 |
Journal | Doklady Biochemistry and Biophysics |
Volume | 494 |
Issue number | 1 |
DOIs | |
State | Published - 1 Sep 2020 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2020, Pleiades Publishing, Ltd.
Funding
This work was supported by the Russian Science Foundation (project no. 17-14-01338). ACKNOWLEDGMENTS
Funders | Funder number |
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Russian Science Foundation | 17-14-01338 |
Keywords
- ANXA13
- HT29
- MLI
- annexin 13
- endocytosis
- intestinal epithelium
- intracellular transport
- ribosome-inactivating protein
- ricin
- viscumin