Abstract
Pseudomonas aeruginosa produces a galactophilic lectin, PA-IL, that resembles P-fimbrial adhesins of uropathogenic Escherichia coli strains in binding to human P blood group antigens. We examined, in the present study, its interaction with pigeon egg white glycoproteins carrying N-glycans with terminal Galα1-4Gal which inhibit the adhesion of P-fimbriae. For comparison, the lectin concanavalin A (Con A) and additional avian egg whites (of hen and quail) were also examined. The results obtained in both hemagglutination inhibition and Western blot analyses showed that PA-IL, unlike Con A, preferentially reacted with the pigeon egg white glycoproteins. These results, which confirmed PA-IL similarity in sugar specificity to E. coli P-fimbriae, demonstrated the advantage of this purified lectin for representing P-type and additional galactophilic microbial adhesins unavailable in purified stable form, in Western blot analyses.
Original language | English |
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Pages (from-to) | 33-36 |
Number of pages | 4 |
Journal | FEMS Immunology and Medical Microbiology |
Volume | 32 |
Issue number | 1 |
DOIs | |
State | Published - Dec 2001 |
Bibliographical note
Funding Information:This study, which is part of Batia Lerrer’s Ph.D. thesis, was supported by the Research Fund of the Bar-Ilan University. The authors thank Ms. S. Victor and A. Goldreich for the skillful typing of this manuscript and Ms. Ella Gindi for the graphic presentation.
Funding
This study, which is part of Batia Lerrer’s Ph.D. thesis, was supported by the Research Fund of the Bar-Ilan University. The authors thank Ms. S. Victor and A. Goldreich for the skillful typing of this manuscript and Ms. Ella Gindi for the graphic presentation.
Funders | Funder number |
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Research Fund of the Bar-Ilan University |
Keywords
- Glycoprotein
- PA-IL lectin
- Pigeon egg
- Pseudomonas aeruginosa
- Western blotting