Interaction of Pseudomonas aeruginosa galactophilic lectin PA-IL with pigeon egg white glycoproteins

Batia Lerrer, Nechama Gilboa-Garber

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Pseudomonas aeruginosa produces a galactophilic lectin, PA-IL, that resembles P-fimbrial adhesins of uropathogenic Escherichia coli strains in binding to human P blood group antigens. We examined, in the present study, its interaction with pigeon egg white glycoproteins carrying N-glycans with terminal Galα1-4Gal which inhibit the adhesion of P-fimbriae. For comparison, the lectin concanavalin A (Con A) and additional avian egg whites (of hen and quail) were also examined. The results obtained in both hemagglutination inhibition and Western blot analyses showed that PA-IL, unlike Con A, preferentially reacted with the pigeon egg white glycoproteins. These results, which confirmed PA-IL similarity in sugar specificity to E. coli P-fimbriae, demonstrated the advantage of this purified lectin for representing P-type and additional galactophilic microbial adhesins unavailable in purified stable form, in Western blot analyses.

Original languageEnglish
Pages (from-to)33-36
Number of pages4
JournalFEMS Immunology and Medical Microbiology
Volume32
Issue number1
DOIs
StatePublished - Dec 2001

Bibliographical note

Funding Information:
This study, which is part of Batia Lerrer’s Ph.D. thesis, was supported by the Research Fund of the Bar-Ilan University. The authors thank Ms. S. Victor and A. Goldreich for the skillful typing of this manuscript and Ms. Ella Gindi for the graphic presentation.

Funding

This study, which is part of Batia Lerrer’s Ph.D. thesis, was supported by the Research Fund of the Bar-Ilan University. The authors thank Ms. S. Victor and A. Goldreich for the skillful typing of this manuscript and Ms. Ella Gindi for the graphic presentation.

FundersFunder number
Research Fund of the Bar-Ilan University

    Keywords

    • Glycoprotein
    • PA-IL lectin
    • Pigeon egg
    • Pseudomonas aeruginosa
    • Western blotting

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