Interaction between β-carotene and lipoxygenase in human skin

Ronit Bar-Natan, Liat Lomnitski, Yossi Sofer, Shoshana Segman, Ishak Neeman, Shlomo Grossman

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


β-Carotene is widely used in skin care therapy. Its effects on skin are unclear, but actions on lipid peroxidation pathways may be an important element of any protection activities it exerts. This study examines the possible effects of β-carotene on enzymatic lipid peroxidation by lipoxygenase in human skin, using in vitro and ex vivo models. The effects of β-carotene on lipid peroxidation in human skin were studied in skin homogenates and in a semi-in vivo model of skin penetration, using [1-14C]-arachidonic acid or [1-14C]-linoleic acid as substrate. When relatively low concentrations (about 0.3 μM) of β-carotene were added to epidermal homogenates, the major metabolites of arachidonic acid (12-hydroxy-cis-5,8,14, trans-10-eicosatetraenoic acid and 15-hydroxy-cis-5,8,11, trans-13-eicosatetraenoic acid) and of linoleic acid (13-hydroxy-cis-9, trans-11-octadeca dienoic acid and 9-hydroxy-trans-10, cis-12-octadeca dienoic acid) were significantly decreased. Following [1-14C]-linoleic acid penetration through the semi in vivo model layers, the skin surface was the main site in which the major linoleate product, 13-hydroxy-cis-9, trans-11-octadeca dienoic acid was detected. Furthermore, its level was inhibited by up to 80%, compared with the control, when β-carotene was added to the system. The data presented in this study suggest possible interactions between β-carotene and human epidermal lipoxygenase. β-carotene may effect lipid peroxidation in human skin, either as a free radical scavenger or as a specific lipoxygenase inhibitor.

Original languageEnglish
Pages (from-to)935-941
Number of pages7
JournalInternational Journal of Biochemistry and Cell Biology
Issue number8
StatePublished - Aug 1996


  • Lipid peroxidation
  • Lipoxygenase
  • Skin
  • β-carotene


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