TY - JOUR
T1 - Inhibition by caltrin of calcium transport into spermatozoa, liver and heart mitochondria
AU - Breitbart, Haim
AU - Wehbie, Robert S.
AU - San Agustin, Jovenal
AU - Lardy, Henry A.
N1 - Funding Information:
This study was supported in part by a grant (AM 10,334) from the National Institutes of Health.
PY - 1990/2/16
Y1 - 1990/2/16
N2 - The calcium-transport inhibitor, caltrin, isolated from bovine seminal fluid inhibits calcium accumulation by bovine epididymal spermatozoa, spermatozoal mitochondria, rat liver mitochondria and beef heart mitochondria. Respiration studies demonstrate a marked stimulation of oxygen consumption by caltrin in filipin-treated spermatozoa and rat liver mitochondria. A biphasic effect of caltrin on rat liver mitochondrial respiration was noted, with stimulation at low caltrin concentrations and inhibition as the concentration of caltrin is increased. The ability of caltrin to uncouple and/or inhibit respiration in filipin-treated spermatozoa and isolated liver mitochondria indicates that inhibition of mitochondrial calcium accumulation by caltrin results from one of these mechanisms. Only a marginal effect of caltrin on respiration of intact spermatozoa was observed; indicating that the plasma membrane is impermeable to this protein. The differential effect of caltrin on respiration of intact and permeabilized spermatozoa indicates that caltrin inhibition of Ca2+ uptake into spermatozoa in vivo occurs at the level of the plasma membrane.
AB - The calcium-transport inhibitor, caltrin, isolated from bovine seminal fluid inhibits calcium accumulation by bovine epididymal spermatozoa, spermatozoal mitochondria, rat liver mitochondria and beef heart mitochondria. Respiration studies demonstrate a marked stimulation of oxygen consumption by caltrin in filipin-treated spermatozoa and rat liver mitochondria. A biphasic effect of caltrin on rat liver mitochondrial respiration was noted, with stimulation at low caltrin concentrations and inhibition as the concentration of caltrin is increased. The ability of caltrin to uncouple and/or inhibit respiration in filipin-treated spermatozoa and isolated liver mitochondria indicates that inhibition of mitochondrial calcium accumulation by caltrin results from one of these mechanisms. Only a marginal effect of caltrin on respiration of intact spermatozoa was observed; indicating that the plasma membrane is impermeable to this protein. The differential effect of caltrin on respiration of intact and permeabilized spermatozoa indicates that caltrin inhibition of Ca2+ uptake into spermatozoa in vivo occurs at the level of the plasma membrane.
KW - Calcium ion transport
KW - Caltrin
KW - Mitochondrion
UR - http://www.scopus.com/inward/record.url?scp=0025016884&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(90)90396-6
DO - 10.1016/0005-2736(90)90396-6
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C2 - 2302400
AN - SCOPUS:0025016884
SN - 0005-2736
VL - 1022
SP - 27
EP - 32
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -