Induction of Growth Arrest by a Temperature-Sensitive p53 Mutant Is Correlated with Increased Nuclear Localization and Decreased Stability of the Protein

Doron Ginsberg, Dan Michael-Michalovitz, Dorit Ginsberg, Moshe Oren

Research output: Contribution to journalArticlepeer-review

172 Scopus citations

Abstract

A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5°C. While much of the protein was cytoplasmic in cells proliferating at 37.5°C, it became predominantly nuclear at 32.5°C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.

Original languageEnglish
Pages (from-to)582-585
Number of pages4
JournalMolecular and Cellular Biology
Volume11
Issue number1
StatePublished - Jan 1991
Externally publishedYes

Funding

FundersFunder number
National Cancer InstituteR01CA040099

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