Induction of acetylcholine receptors in muscle cultures

A. Shainberg, S. A. Cohen, P. G. Nelson

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Acetylcholine receptors in muscle cells differentiated in vitro were monitored by using125I-α-bungarotoxin. The number of cholinergic receptors was increased 4-8 fold in 2 days due to inhibition of spontaneous contraction of the muscle fibers. The inhibition of this activity, whether mediated through tetrodotoxin, lidocaine or D-600, did not affect the biochemical differentiation of muscle, as represented by creatine-phosphokinase and acetylcholinesterase activity. "Induction" of receptors by tetrodotoxin was inhibited by cycloheximide, actinomycin-D, or 5-bromotubercidine. Dystrophic muscle responded in vitro to inhibition of contraction similar to normal tissue.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Issue number3
StatePublished - Jan 1976


  • Acetylcholine receptors
  • Alpha bungarotoxin
  • Denervation hypersensitivity
  • Tetrodotoxin


Dive into the research topics of 'Induction of acetylcholine receptors in muscle cultures'. Together they form a unique fingerprint.

Cite this