Increased expression of the hPim-2 gene in human chronic lymphocytic leukemia and non-Hodgkin lymphoma

Amos M. Cohen, Borislava Grinblat, Hanna Bessler, Donald A. Kristt, Alona Kremer, Sarah Shalom, Ariel Schwartz, Marisa Halperin, Drorit Merkel, Jeremy Don

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104 Scopus citations

Abstract

Pim-1 and Pim-2 are murine proto-oncogenes implicated in lymphomagenesis. The aim of this study was to investigate whether the human Pim-2 (hPim-2) expression is altered in chronic lymphocytic leukemia (B-CLL) and non-Hodgkin's lymphomas (NHL). We analyzed hPim-2 expression in 48 patients with NHL and CLL by quantitative in-situ hybridization, quantitative RT - PCR and FACS analysis. In-situ hybridization revealed a 5.5 ± 2.2 times higher expression of hPim-2 in NHL over normal lymphocytes (P < 0.001). Similarly, with quantitative RT-PCR, expression in NHL was 1.5 to 2.6 times higher in involved splenic foci compared to nearby uninvolved regions (n = 3). hPim-2 m RNA was increased 3-folds in B-CLL over normal B-cells (P < 0.006). The increased hPim-2 levels correlated with lymphocyte doubling time (DT), in that mRNA levels were two times greater in patients with rapid DT (P < 0.006). Moreover, a significant correlation was found between hPim-2 expression and the Binet staging system of CLL (P < 0.022). The hPIM-2 protein expression was also upregulated in CLL, as assessed by FACS analysis. Therefore, this report provides direct evidence for a linkage of hPim-2 upregulation to NHL and CLL in man. This relationship between hPim-2 and NHL and CLL raises a number of novel mechanistic options for the genesis and/or progression of some types of human lymphomas.

Original languageEnglish
Pages (from-to)951-955
Number of pages5
JournalLeukemia and Lymphoma
Volume45
Issue number5
DOIs
StatePublished - May 2004

Bibliographical note

Funding Information:
This research was supported in part by grants from the Israel Cancer Association (20000102-B), the Israel Ministry of Health (200/2000), the Rabin Medical Center Research Fund and the Meirbaum Fund, Tel-Aviv University.

Funding

This research was supported in part by grants from the Israel Cancer Association (20000102-B), the Israel Ministry of Health (200/2000), the Rabin Medical Center Research Fund and the Meirbaum Fund, Tel-Aviv University.

FundersFunder number
Israel Ministry of Health200/2000
Meirbaum Fund
Rabin Medical Center Research Fund
Tel-Aviv University
Israel Cancer Association20000102-B

    Keywords

    • B-CLL
    • Gene expression
    • Human Pim-2
    • Non-Hodgkin's lymphoma

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