Inactivation of erythrocytic, lymphocytic and myelocytic leukemic cells by photoexcitation of endogenous porphyrins

Zvi Malik; Benjamin Ehrenberg; Ariela Faraggi

doi:10.1016/1011-1344(89)80005-3

Inactivation of erythrocytic, lymphocytic and myelocytic leukemic cells by photoexcitation of endogenous porphyrins

Zvi Malik, Benjamin Ehrenberg, Ariela Faraggi

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

The photodynamic sensitization of leukemic cells (erythrocytic, myelocytic and lymphocytic) via light activation of endogenous porphyrins is described. Human myelocytic-erythrocytic K562 cells and murine Friend erythroleukemia (FELC) and T-cell lymphoma Eb-Esb cells were stimulated to synthesize and accumulate porphyrins. K562 cells accumulated high amounts of protoporphyrin by stimulation with 5-aminolevulinic acid (ALA) plus sodium butyrate or hemin. For Friend and Eb-Esb cells ALA was an adequate stimulator. The high-metastatic Esb lymphoma cells accumulated comparatively more porphyrin than the low-metastatic Eb cell line. Maximal porphyrin accumulation produced mortality rates of more than 99% after 10 min of photoactivation of the three leukemic lines. Thymidine incorporation was inhibited by the photodynamic effect depending on porphyrin concentration. These results confirm the photodynamic ability of endogenous porphyrins to inactivate cancer cells of different origins.

Original language	English
Pages (from-to)	195-205
Number of pages	11
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/1011-1344(89)80005-3
State	Published - Nov 1989

Bibliographical note

Funding Information:
This research was supported by a grant to Z.M. and B.E. from the Ministry of Health, state of Israel and by grant to Z.M. from the Health Sciences Center of the Bar-Ilan University. The authors wish to thank Mrs. J. Hanania for skillful and excellent technical assistance.

Funding

This research was supported by a grant to Z.M. and B.E. from the Ministry of Health, state of Israel and by grant to Z.M. from the Health Sciences Center of the Bar-Ilan University. The authors wish to thank Mrs. J. Hanania for skillful and excellent technical assistance.

Funders	Funder number
Health Sciences Center of the Bar-Ilan University
Ministry of Health, State of Israel

Keywords

Porphyrins
aminolevulinic acid.
leukemia
photodynamic therapy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/1011-1344(89)80005-3

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title = "Inactivation of erythrocytic, lymphocytic and myelocytic leukemic cells by photoexcitation of endogenous porphyrins",

abstract = "The photodynamic sensitization of leukemic cells (erythrocytic, myelocytic and lymphocytic) via light activation of endogenous porphyrins is described. Human myelocytic-erythrocytic K562 cells and murine Friend erythroleukemia (FELC) and T-cell lymphoma Eb-Esb cells were stimulated to synthesize and accumulate porphyrins. K562 cells accumulated high amounts of protoporphyrin by stimulation with 5-aminolevulinic acid (ALA) plus sodium butyrate or hemin. For Friend and Eb-Esb cells ALA was an adequate stimulator. The high-metastatic Esb lymphoma cells accumulated comparatively more porphyrin than the low-metastatic Eb cell line. Maximal porphyrin accumulation produced mortality rates of more than 99% after 10 min of photoactivation of the three leukemic lines. Thymidine incorporation was inhibited by the photodynamic effect depending on porphyrin concentration. These results confirm the photodynamic ability of endogenous porphyrins to inactivate cancer cells of different origins.",

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note = "Funding Information: This research was supported by a grant to Z.M. and B.E. from the Ministry of Health, state of Israel and by grant to Z.M. from the Health Sciences Center of the Bar-Ilan University. The authors wish to thank Mrs. J. Hanania for skillful and excellent technical assistance.",

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AU - Ehrenberg, Benjamin

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N2 - The photodynamic sensitization of leukemic cells (erythrocytic, myelocytic and lymphocytic) via light activation of endogenous porphyrins is described. Human myelocytic-erythrocytic K562 cells and murine Friend erythroleukemia (FELC) and T-cell lymphoma Eb-Esb cells were stimulated to synthesize and accumulate porphyrins. K562 cells accumulated high amounts of protoporphyrin by stimulation with 5-aminolevulinic acid (ALA) plus sodium butyrate or hemin. For Friend and Eb-Esb cells ALA was an adequate stimulator. The high-metastatic Esb lymphoma cells accumulated comparatively more porphyrin than the low-metastatic Eb cell line. Maximal porphyrin accumulation produced mortality rates of more than 99% after 10 min of photoactivation of the three leukemic lines. Thymidine incorporation was inhibited by the photodynamic effect depending on porphyrin concentration. These results confirm the photodynamic ability of endogenous porphyrins to inactivate cancer cells of different origins.

AB - The photodynamic sensitization of leukemic cells (erythrocytic, myelocytic and lymphocytic) via light activation of endogenous porphyrins is described. Human myelocytic-erythrocytic K562 cells and murine Friend erythroleukemia (FELC) and T-cell lymphoma Eb-Esb cells were stimulated to synthesize and accumulate porphyrins. K562 cells accumulated high amounts of protoporphyrin by stimulation with 5-aminolevulinic acid (ALA) plus sodium butyrate or hemin. For Friend and Eb-Esb cells ALA was an adequate stimulator. The high-metastatic Esb lymphoma cells accumulated comparatively more porphyrin than the low-metastatic Eb cell line. Maximal porphyrin accumulation produced mortality rates of more than 99% after 10 min of photoactivation of the three leukemic lines. Thymidine incorporation was inhibited by the photodynamic effect depending on porphyrin concentration. These results confirm the photodynamic ability of endogenous porphyrins to inactivate cancer cells of different origins.

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Inactivation of erythrocytic, lymphocytic and myelocytic leukemic cells by photoexcitation of endogenous porphyrins

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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